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| Status |
Public on Jun 03, 2024 |
| Title |
Effects of Klf4 knockdown in GT1-7 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal is to examine the relative abundance of Agrp transcripts and changes to gene expression within GT1-7 cells (immortalized mouse hypothalamic cell line, used as a model of AgRP neurons) treated with Klf4 siRNA, scramble siRNA, or Ca2+ DMEM (vehicle).
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| |
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| Overall design |
Exon count and differential gene expression analyses from RNA-seq data from GT1-7 cells comparing a Klf4 siRNA, scramble siRNA and a Ca2+ free vehicle group
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| |
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| Contributor(s) |
Ritter M, Grobe J |
| Citation(s) |
38145289 |
| |
| Submission date |
Feb 07, 2023 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Justin L Grobe |
| E-mail(s) |
jgrobe@mcw.edu
|
| Organization name |
Medical College of Wisconsin
|
| Street address |
8701 W Watertown Plank Road
|
| City |
Milwaukee |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA932266 |
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