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| Status |
Public on Mar 09, 2023 |
| Title |
Network-based elucidation of colon cancer drug resistance by phosphoproteomic time-series analysis |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Aberrant signaling pathway activity is a hallmark of tumorigenesis and progression, which has guided targeted inhibitor design for over 30 years. Yet, adaptive resistance mechanisms, induced by rapid, context-specific signaling network rewiring, continue to challenge therapeutic efficacy. By leveraging progress in proteomic technologies and network-based methodologies over the past decade we developed VESPA—an algorithm designed to elucidate mechanisms of cell response and adaptation to drug perturbations—and used it to analyze 7-point phosphoproteomic time series from colorectal cancer cells treated with clinically-relevant inhibitors and control media. Interrogation of tumor-specific enzyme/substrate interactions accurately inferred kinase and phosphatase activity, based on their inferred substrate phosphorylation state, effectively accounting for signal cross-talk and sparse phosphoproteome coverage. The analysis elucidated time-dependent signaling pathway response to each drug perturbation and, more importantly, cell adaptive response and rewiring that was experimentally confirmed by CRISPRko assays, suggesting broad applicability to cancer and other diseases.
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| Overall design |
This experiment represents a large-scale, pooled CRISPR knockout (CRISPRko) screen validtion experiment, targeting all annotated human kinases, phosphatases and E3 ligases (Methods) of two human colorectal cancer cell lines HCT-15 and NCI-H508, perturbed with two drug compounds (linsitib, trametinib). Data was measured with four different guides per target.
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| Contributor(s) |
Rosenberger G, Turunen M, Griffin AT, Califano A |
| Citation(s) |
36824919 |
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| Submission date |
Feb 02, 2023 |
| Last update date |
Mar 09, 2023 |
| Contact name |
Andrea Califano |
| E-mail(s) |
ac2248@cumc.columbia.edu
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| Organization name |
Califano Laboratory of Systems Biology
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| Department |
Department of Systems Biology
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| Lab |
Cal
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| Street address |
1130 St. Nicholas Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA930770 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224396_Kin_Phos_E3_sgRNA_seqs.xlsx |
175.9 Kb |
(ftp)(http) |
XLSX |
| GSE224396_stable17_deseq2_sgrna.csv.gz |
163.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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