GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE22430 Query DataSets for GSE22430
Status Public on Jun 01, 2011
Title Inflammatory activation of airway epithelial cells by pyocyanin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Despite the appreciated in vivo role of the redox-active Pseudomonas virulence factor pyocyanin in Pseudomonas airway infections and the recognized importance of airway epithelial cells in combating bacterial pathogens, little is known about pyocyanin’s effect on airway epithelial cells. We find that exposure of bronchiolar epithelial cells to pyocyanin results in MUC2/MUC5AC induction and mucin secretion mediated by reactive oxygen species production, activation of the epidermal growth factor receptor pathway and release of inflammatory cytokines (IL-1b, IL-6, TGFa, TNFa). Microarray analysis identified 286 pyocyanin-induced genes in airway epithelial cells, including many of the inflammatory mediators elevated in cystic fibrosis airways (G-CSF, GM-CSF, CXCL1, SAA). We also found several novel pyocyanin-responsive genes of potential importance in the infection process (IL-24, CXCL2, CXCL3, CCL20, SOD2). This comprehensive study uncovers numerous details of pyocyanin’s proinflammatory action and establishes airway epithelial cells as key responders following exposure to this microbial toxin.
Overall design H292 cells were treated with or without 8 uM pyocyanin for 48 hrs in serum-free RPMI medium. RNA was isolated in four independent experiments, collected, processed and subjected to microarray analysis simultaneously (biological repeats).
Citation(s) 20962773
Submission date Jun 17, 2010
Last update date Feb 22, 2018
Contact name Balazs Rada
Phone 301 4356534
Fax 301 4801731
Organization name NIH
Department NIAID
Lab Lab of Host Defenses
Street address 12441 Parklawn Drive
City Rockville
State/province MD
ZIP/Postal code 20852
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (4)
GSM557479 PYO Treated - repeat 1 - mAdbID:103983
GSM557480 PYO Treated - repeat 2 - mAdbID:103984
GSM557481 PYO Treated - repeat 3 - mAdbID:103985
BioProject PRJNA128555

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22430_RAW.tar 69.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap