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| Status |
Public on Jun 01, 2011 |
| Title |
Inflammatory activation of airway epithelial cells by pyocyanin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Despite the appreciated in vivo role of the redox-active Pseudomonas virulence factor pyocyanin in Pseudomonas airway infections and the recognized importance of airway epithelial cells in combating bacterial pathogens, little is known about pyocyanin’s effect on airway epithelial cells. We find that exposure of bronchiolar epithelial cells to pyocyanin results in MUC2/MUC5AC induction and mucin secretion mediated by reactive oxygen species production, activation of the epidermal growth factor receptor pathway and release of inflammatory cytokines (IL-1b, IL-6, TGFa, TNFa). Microarray analysis identified 286 pyocyanin-induced genes in airway epithelial cells, including many of the inflammatory mediators elevated in cystic fibrosis airways (G-CSF, GM-CSF, CXCL1, SAA). We also found several novel pyocyanin-responsive genes of potential importance in the infection process (IL-24, CXCL2, CXCL3, CCL20, SOD2). This comprehensive study uncovers numerous details of pyocyanin’s proinflammatory action and establishes airway epithelial cells as key responders following exposure to this microbial toxin.
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| Overall design |
H292 cells were treated with or without 8 uM pyocyanin for 48 hrs in serum-free RPMI medium. RNA was isolated in four independent experiments, collected, processed and subjected to microarray analysis simultaneously (biological repeats).
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| Citation(s) |
20962773 |
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| Submission date |
Jun 17, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Balazs Rada |
| E-mail(s) |
radab@mail.nih.gov
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| Phone |
301 4356534
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| Fax |
301 4801731
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| Organization name |
NIH
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| Department |
NIAID
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| Lab |
Lab of Host Defenses
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| Street address |
12441 Parklawn Drive
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| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20852 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| GSM557479 |
PYO Treated - repeat 1 - mAdbID:103983 |
| GSM557480 |
PYO Treated - repeat 2 - mAdbID:103984 |
| GSM557481 |
PYO Treated - repeat 3 - mAdbID:103985 |
| GSM557482 |
PYO Treated - repeat 4 - mAdbID:103986 |
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| Relations |
| BioProject |
PRJNA128555 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22430_RAW.tar |
69.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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