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| Status |
Public on Mar 17, 2023 |
| Title |
Comparison of different T cell differentiation states by RNA-seq analysis identifies BX-795 induced Th-IL-2 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We here report RNA-seq data on CD4+ T cells from double transgenic allergy-mice (Neunkirchner A. et al.) expressing a humanized T cell receptor (TCR) specific for the major mugwort pollen allergen Art v 1 peptide 23-36 in combination with a humanized HLA-DR molecule at time-point zero (FACS sorted naive CD4+ T cells from murine spleen) and after 120 h of stimulation of such FACS sorted naive T cells with bone-marrow derived dendritic cells (BMDCs) from these mice plus recombinant Art v 1 (rArt v 1) protein and either DMSO (T effector), BX-795 (Th-IL-2) or a mix of TGF-b, anti-IL-4 and anti-IFN-g (iTreg). T cells cultured for 120 h were FACS sorted before RNA extraction to exclude BMDCs (purity > 90 %). These data is supposed to characterize the expression phenotype of T cells differentiated in the presence of BX-795 (Th-IL-2) and extract specific features by comparing it to control phenotypes (naive, T effector, iTreg).
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| Overall design |
RNA-seq of murine naive CD4+ T cells at time point 0 and after 120 h of stimulation at various differentiation conditions
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| Contributor(s) |
Tauber PA, Pickl WF |
| Citation(s) |
37090735 |
| |
| Submission date |
Feb 01, 2023 |
| Last update date |
May 12, 2023 |
| Contact name |
Winfried F Pickl |
| Organization name |
Medical University of Vienna
|
| Street address |
Lazarettgasse 19
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA930436 |
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