NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE223991 Query DataSets for GSE223991
Status Public on Sep 28, 2023
Title CRISPR Anchor screening of a barcoded library in cells treated with standard of care neuroblastoma drugs
Organism Homo sapiens
Experiment type Other
Summary Neuroblastoma cells were treated with a barcoded library of >600 genes and one of several standrard of care neuroblastoma compounds (or vehicle control)
 
Overall design Sequencing of gRNAs of each cell line treated with the barcoded library and a standard of care compound or vehicle control
The sequencing data (gRNA abundance data in drug-treated cells) are analyzed with a de facto tool called MAGeCK. This tool is standard and outputs data for all measured gRNA abundances for all perturbation conditions (drug treatments in our case) for all samples. The detailed description can be found at https://sourceforge.net/p/mageck/wiki/output/
 
Contributor(s) Wright WC
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 29, 2023
Last update date Nov 03, 2023
Contact name William C. Wright
E-mail(s) charlie.wright@stjude.org
Organization name St. Jude Children's Research Hospital
Department Computational Biology
Street address 262 Danny Thomas Pl
City Memphis
State/province Tennessee
ZIP/Postal code 38105
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (202)
GSM7009327 pool CHP134 cells treated with CX-5461 rep1
GSM7009328 pool CHP134 cells treated with CX-5461 rep2
GSM7009329 pool CHP134 cells treated with Veh72
Relations
BioProject PRJNA929273

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223991_Master_Processed_Data.csv.gz 5.4 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap