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| Status |
Public on Jan 31, 2024 |
| Title |
ChIP-seq using BrJMJ18-OX Par plants under NC and HS conditions |
| Organism |
Brassica rapa var. parachinensis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Using genome-wide pattern analysis and quantitative-trait-locus (QTL) mapping, we identified that a natural allele of BrJMJ18, BrJMJ18Par, encoding a novel H3K36me3/2 Jumonji demethylase, confers thermotolerance by delayed flowering and stimulated vegetative growth on B. rapa.To assess the downstream genes of BrJMJ18, we conducted a chromatin immunoprecipitation (ChIP) seq experiment using a polyclonal antibody recognizing BrJMJ18 in Par plants under normal conditon (LD, 21℃) and high temperature condiation (LD, 28℃), respectively.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for BrJMJ18-GFP in BrJMJ18 overexpresing Par plants. Par plants overexpressing tow allels of BrJMJ18, BrJMJ18WT (H1) and BrJMJ18Par (H2) were grown under low temperature (L, 21℃) for 5 weeks or low temperature (L, 21℃) for 4 weeks following with 1 week under high temperature (H, 28℃), respectively. And wildtype Par plant was used as control (CK). The young and health leaves were collected for ChIP-seq. Two biological replicates were performed for each sample.
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| Contributor(s) |
Su T, Xin X, Li P, Yu S |
| Citation missing |
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| Submission date |
Jan 29, 2023 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Peirong Li |
| E-mail(s) |
lprdream@126.com
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| Organization name |
Beijing Academy of Agricultural and Forestry Sciences
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| Street address |
Zhanghualu No.50
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100097 |
| Country |
China |
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| Platforms (1) |
| GPL33070 |
Illumina NovaSeq 6000 (Brassica rapa var. parachinensis) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA929226 |
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