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Series GSE223623 Query DataSets for GSE223623
Status Public on Jan 31, 2023
Title ShRNA screen identifies novel mediator of PDAC metastasis in vivo
Organism Mus musculus
Experiment type Other
Summary An shRNA screen with the aim to investigate the role of pancreatic cancer-associated genes in the metastatic outgrowth in vivo was carried out. The set of candidate genes (284) was selected based on their association with pancreatic cancer, frequency of mutation in pancreatic cancer or on being Ras-regulated.
 
Overall design The abundance of shRNAs was assessed in cells prior to injection (Day 0), in isolated lung tumours (Day 28), and in cells grown in culture (Day 28). Each gene was targeted with 3-4 shRNA producing a library size of 1013 shRNA. Nineteen shRNA pools were generated and each contained 8 control shRNA to assess consistency across pools.
 
Contributor(s) Sheridan C, Horswell S, Downward J
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Submission date Jan 24, 2023
Last update date Feb 06, 2023
Contact name Goran Tomic
Organization name The Francis Crick Institute
Lab Julian Downward
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (158)
GSM6965751 pool 3A pool number 3
GSM6965752 pool Cells 13A pool number 13
GSM6965753 pool 9B pool number 9
Relations
BioProject PRJNA926988

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223623_RAW.tar 610.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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