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| Status |
Public on Jan 28, 2023 |
| Title |
Expression data from MDA-MB-231 breast cancer cell lines with DAXX overexpression (OE) and DAXX SUMO interacting motif mutant (DSM) overexpression (DSM OE) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
DAXX was originally discovered as a context-dependent regulator of cell death or survival. Emerging evidence suggests that DAXX has an oncogenic role in diverse cancer types. However, the molecular mechanisms underlying DAXX's oncogenic function remain to be defined. We used Affymetrix Human Transcriptome Array 2.0 (HTA-2_0) to analyze genes that were up or downregulated upon DAXX OE and DSM OE.
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| Overall design |
Both DAXX OE and DSM OE MDA-MB-231 cells were seeded at 2 x 10^5 cells in 10 cm culture plate. Stable expression of cDNA was established through lentiviral transduction of MDA-MB-231 cell line and puromycin (2 µg/mL) selection. Three biological replicates were applied.
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| Contributor(s) |
Mahmud I, Liao D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 24, 2023 |
| Last update date |
Jan 30, 2023 |
| Contact name |
Daiqing Liao |
| E-mail(s) |
dliao@ufl.edu
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| Phone |
(352) 273-8188
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| Organization name |
University of Florida
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| Department |
Anatomy and Cell Biology
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| Lab |
480E
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| Street address |
2033 Mowry Rd
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA926921 |
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