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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 15, 2010 |
| Title |
Expression data from cones in degenerated retinas from C3H/HeNCrl (Pde6brd1) mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used FACS isolated RD cone photoreceptors from C3H mice (we refer this mouse model as f-RD) that were transfected by AAVs to express fluorescent reporters to genomic analyses. We tested three different ages.
Many cone photoreceptor markers are highly enriched, identifying the isolated RD cones as cones.
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| Overall design |
Two different treatments: mCAR-EGFP or mCAR-eNpHR-EYFP, where mCAR-EGFP served as a control condition and mCAR-eNpHR-EYFP was the light sensor.
Mouse cone arrestin-3 promoter (mCAR) was used to drive EGFP/eNpHR-EYFP in RD photoreceptors. Cones are brightly labeled by these constructs, indicating that the intrinsic promoter is active and intrinsic cone markers are expressed.
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| Contributor(s) |
Busskamp V, Cabuy E |
| Citation(s) |
20576849 |
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| Submission date |
Jun 14, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Erik Cabuy |
| E-mail(s) |
erik.cabuy@fmi.ch
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| Phone |
+41 616976895
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| URL |
http://www.fmi.ch
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| Organization name |
Friedrich Miescher Institute
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| Department |
Single Cell Genomics
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| Lab |
WRO-1066.1.58
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| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA127513 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22338_RAW.tar |
40.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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