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Series GSE223282 Query DataSets for GSE223282
Status Public on Jan 31, 2024
Title Use-dependent synaptic plasticity associates with modulation of blood-brain-barrier permeability in the somatosensory cortex
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary The effect of neuronal activity on blood-brain barrier function and whether it plays a role in plasticity in the healthy brain remains unclear. We show that neuronal activity induces modulation of microvascular permeability in the healthy brain and that it has a role in local network reorganization. Combining simultaneous electrophysiological recording and vascular imaging with transcriptomic analysis in rats, and functional and BBB-mapping MRI in human subjects we show that prolonged stimulation of the limb induces a focal increase in BBB permeability in the corresponding somatosensory cortex that is associated with long-term synaptic plasticity. We further show that the increased microvascular permeability depends on neuronal activity and involves caveolae-mediated transcytosis and transforming growth factor beta signaling. Our results reveal a role of BBB modulation in cortical plasticity in the healthy brain, highlighting the importance of neurovascular interactions for sensory experience and learning.
 
Overall design Bulk RNA-sequencing of rat sensorimotor cortex contralateral and ipsilateral to the stimulated limb. Tissue was harvested 1 hour and 24 hour post-stimulation for transcriptomic analysis of differentially expressed genes in the contralateral (“stimulated”) compared to the ipsilateral (“non-stimulated”) cortex. Corresponding striatum samples were likewise dissected and sequenced for RNA-sequencing validation and reference control.
 
Contributor(s) Yang LT, Swissa E, Kaufer D, Friedman A
Citation(s) 39024007
Submission date Jan 19, 2023
Last update date Aug 09, 2024
Contact name Lynn T. Yang
Organization name UC Berkeley
Department Integrative Biology, Helen Wills Neuroscience Institute
Lab Kaufer Lab
Street address 1951 Oxford Street, 430E
City Berkeley
State/province CA
ZIP/Postal code 94709
Country USA
 
Platforms (1)
GPL25947 Illumina NovaSeq 6000 (Rattus norvegicus)
Samples (40)
GSM6944394 sample1_contralateral sensorimotor cortex, 1 hour
GSM6944395 sample2_contralateral sensorimotor cortex, 1 hour
GSM6944396 sample3_contralateral sensorimotor cortex, 1 hour
Relations
BioProject PRJNA925512

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223282_DESeq2normalized_counts.txt.gz 4.3 Mb (ftp)(http) TXT
GSE223282_raw_featureCounts.txt.gz 2.5 Mb (ftp)(http) TXT
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