NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE222744 Query DataSets for GSE222744
Status Public on Jan 12, 2024
Title The clock gene Per1 may exert diurnal control over hippocampal memory consolidation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The circadian system influences many different biological processes, including memory performance. While the suprachiasmatic nucleus (SCN) functions as the brain’s central pacemaker, downstream “satellite clocks” may also regulate local functions based on the time of day. Within the dorsal hippocampus (DH), for example, local molecular oscillations may contribute to time-of-day effects on memory. Here, we used the hippocampus-dependent Object Location Memory task to determine how memory is regulated across the day/night cycle in mice. First, we systematically determined which phase of memory (acquisition, consolidation, or retrieval) is modulated across the 24h day. We found that mice show better long-term memory performance during the day than at night, an effect that was specifically attributed to diurnal changes in memory consolidation, as neither memory acquisition nor memory retrieval fluctuated across the day/night cycle. Using RNA-sequencing we identified the circadian clock gene Period1 (Per1) as a key mechanism capable of supporting this diurnal fluctuation in memory consolidation, as Per1 oscillates in tandem with memory performance. We then show that local knockdown of Per1 within the DH has no effect on either the circadian rhythm or sleep behavior, although previous work has shown this manipulation impairs memory. Thus, Per1 may independently function within the DH to regulate memory in addition to its known role in regulating the circadian system within the SCN. Per1 may therefore exert local diurnal control over memory consolidation within the DH.
 
Overall design Differential gene expression analyses of RNA-seq data from dorsal hippocampus punches for naive homecage (HC) mice compare to mice trained with object location memory (OLM) at 6 diurnal timepoints (ZT1, ZT5, ZT9, ZT13, ZT17, and ZT21) and sacrificed 60m after training.
 
Contributor(s) Bellfy L, Smies CW, Bernhardt AR, Bodinayake KK, Sebastian A, Lo C, Murakami S, Boyd HM, von Abo MJ, Albert I, Kwapis JL
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 12, 2023
Last update date Jan 12, 2024
Contact name Aswathy Sebastian
E-mail(s) azs13@psu.edu
Phone 5204613475
Organization name PennState
Street address University Park
City State College
ZIP/Postal code 16801
Country USA
 
Platforms (1)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (72)
GSM6930060 ZT1, OLM [G610_01_RS1_S1]
GSM6930061 ZT1, OLM [G610_25_RS3_S25]
GSM6930062 ZT1, OLM [G610_02_RS73_S2]
Relations
BioProject PRJNA923300

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE222744_gene_counts.txt.gz 6.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap