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| Status |
Public on Jun 30, 2011 |
| Title |
Expression data from Saccharomyces cerevisiae temperature-sensitive strains specific for SCF core components (Skp1, Cullin (Cdc53), E2 enzyme (Cdc34)) and the F-box protein Cdc4. |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable.
We used a yeast-based high-throughput chemical genetic screen to identify small-molecule enhancers of rapamycin (SMERs) and used whole genome expression analysis to identify their mechanisms of action.
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| Overall design |
We incubated various temperature-sensitive yeast strains at either the permissive (room temperature) or non-permissive (35°C) temperature for 1 hour prior to RNA extraction and hybridization on Affymetrix microarrays. Comparison of expression profiles enabled identification of gene-signatures specific to inhibition of each gene product.
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| Contributor(s) |
Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 10, 2010 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Mariam Aghajan |
| Organization name |
UCLA
|
| Street address |
650 Charles E. Young Drive South, CHS 23-231
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE22272 |
Chemical Genetics of TOR Identifies an SCF Family E3 Ubiquitin Ligase Inhibitor |
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| Relations |
| BioProject |
PRJNA129253 |
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