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| Status |
Public on Sep 23, 2024 |
| Title |
Fecal microbiota in mice before and after repeated mild stress |
| Organism |
feces metagenome |
| Experiment type |
Other
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| Summary |
To examine potential changes of the intestinal microbiota in mice caused by repeated mild stress, we profiled bacteria and fungi in the mouse feces by sequencing the 16s v3v4 region and the ITS1-2 region.
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| Overall design |
Mice feces were collected before and after stress. Fecal microbial DNA were extracted by using QIAamp PowerFecal Pro DNA Kit (Cat. 51804). The 16s v3v4 regions and ITS1-2 regions were amplified by PCR with barcoded primers and sequenced with 2×300 paired-end reading. Data analysis were carried out with QIIME2(v2019.7)
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| Contributor(s) |
Yu X, Chen L, Qi Q, Jin Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 10, 2023 |
| Last update date |
Sep 23, 2024 |
| Contact name |
Xiaofei Yu |
| E-mail(s) |
xiaofei_yu@fudan.edu.cn
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| Organization name |
Fudan University
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| Street address |
2005 Songhu Rd
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| City |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
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| Platforms (1) |
| GPL24116 |
Illumina MiSeq (feces metagenome) |
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| Samples (28)
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| GSM6925651 |
Mouse11, 16s v3v4, Before Stress |
| GSM6925652 |
Mouse12, 16s v3v4, Before Stress |
| GSM6925653 |
Mouse13, 16s v3v4, Before Stress |
| GSM6925654 |
Mouse14, 16s v3v4, Before Stress |
| GSM6925655 |
Mouse8, 16s v3v4, After Stress |
| GSM6925656 |
Mouse9, 16s v3v4, After Stress |
| GSM6925657 |
Mouse10, 16s v3v4, After Stress |
| GSM6925658 |
Mouse11, 16s v3v4, After Stress |
| GSM6925659 |
Mouse12, 16s v3v4, After Stress |
| GSM6925660 |
Mouse13, 16s v3v4, After Stress |
| GSM6925661 |
Mouse14, 16s v3v4, After Stress |
| GSM6925662 |
Mouse8, ITS1-2, Before Stress |
| GSM6925663 |
Mouse9, ITS1-2, Before Stress |
| GSM6925664 |
Mouse10, ITS1-2, Before Stress |
| GSM6925665 |
Mouse11, ITS1-2, Before Stress |
| GSM6925666 |
Mouse12, ITS1-2, Before Stress |
| GSM6925667 |
Mouse13, ITS1-2, Before Stress |
| GSM6925668 |
Mouse14, ITS1-2, Before Stress |
| GSM6925669 |
Mouse8, ITS1-2, After Stress |
| GSM6925670 |
Mouse9, ITS1-2, After Stress |
| GSM6925671 |
Mouse10, ITS1-2, After Stress |
| GSM6925672 |
Mouse11, ITS1-2, After Stress |
| GSM6925673 |
Mouse12, ITS1-2, After Stress |
| GSM6925674 |
Mouse13, ITS1-2, After Stress |
| GSM6925675 |
Mouse14, ITS1-2, After Stress |
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| Relations |
| BioProject |
PRJNA922482 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222533_16s_asv_table.tsv.gz |
6.2 Kb |
(ftp)(http) |
TSV |
| GSE222533_its_asv_table.tsv.gz |
7.6 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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