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| Status |
Public on Jan 06, 2023 |
| Title |
Examining the Effect of Notocactus ottonis Cold Vacuum Isolated Cell Extract on Hair Growth in C57BL/6 Mice using a Combination of Physiology and OMICS analyses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study focuses on the hair-growing effects of a cell extract (CE) obtained from the cactus Notocactus ottonis by a cold vacuum extraction protocol, with the aim of investigating its hair-growing effects and relevant mechanisms and potential factors therein. In an experiment using male C57BL/6 mice, a vehicle control group (VC: propylene glycol : ethanol : water), a positive control group (MXD : 3% minoxidil), and an experimental group (N-CE: 10% N. ottonis CE) were applied topically to the backs of mice. Results showed that 3% MXD and 10% N-CE were more effective in promoting hair growth than the VC. Furthermore, an increase in the number of hair follicles was observed with 10% N-CE in hematoxylin-eosin-stained skin tissue.
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| Overall design |
Whole genome DNA microarray was carried out on the skin samples (the back whole skin tissue of C57BL/6J mice was ground into a fine powder in liquid nitrogen and stored at -80°C until RNA isolation, and total RNA was extracted from powdered mouse skin tissue using the RNeasy Mini Kit ) to suggest molecular factors underlying the hair-promoting effects of N-CE. Total RNA extracted from mouse skin tissue for each control and treatment (n=6) was pooled in each group, prior to DNA microarray analysis.
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| Contributor(s) |
Shibato J, Takenoya F, Kimura A, Yamashita M, Rakwal R, Shioda S |
| Citation(s) |
36838553 |
| |
| Submission date |
Jan 05, 2023 |
| Last update date |
Jun 14, 2023 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
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| Phone |
+81-(0)90-1853-7875
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| Organization name |
University of Tsukuba
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| Department |
Institute of Health and Sport Sciences
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| Lab |
Global Sport Innovation (GSI) 403
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| Street address |
1-1-1 Tennodai
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA918610 |
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