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| Status |
Public on Dec 27, 2022 |
| Title |
Next generation sequencing comparing the gene expression between ICSI and SCNT blastocysts of monkey [RNA-seq] |
| Organism |
Macaca fascicularis x Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys (Macaca fascicularis), but the cloning of other non-human primate species remains to be achieved. On the other hand, our histological examination indicated severe calcification of the placenta of SCNT fetuses. Additionally, we found that some of the maternal-biased imprinting genes were aberrantly lost in the cloned embryos by multi-omics analysis. We thus developed a trophoblast replacement (TR) method, providing an ICSI-derived placenta for the SCNT fetus, to support the full-term development of the cloned embryos after implantation. By combining this TR method with treatment with epigenetic modification factors, we obtained a healthy somatic cell cloned rhesus monkey. Thus, TR represents a useful approach for rhesus monkey cloning.
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| |
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| Overall design |
Whole genome bisulfite sequencing and mRNA sequencing of monkey ICSI and SCNT blastocysts, transcriptome sequencing of monkey ICSI and SCNT placentas
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| |
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| Contributor(s) |
Liao Z, Zhang J |
| Citation(s) |
38228612 |
| |
| Submission date |
Dec 22, 2022 |
| Last update date |
Jan 29, 2024 |
| Contact name |
Liao Zhaodi |
| E-mail(s) |
zhaodiliao@ion.ac.cn
|
| Phone |
17521632708
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Neuroscience
|
| Street address |
500, Qiangye Road
|
| City |
Shanghai |
| ZIP/Postal code |
2016000 |
| Country |
China |
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| Platforms (1) |
| GPL32973 |
Illumina NovaSeq 6000 (Macaca fascicularis x Macaca mulatta) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA915100 |
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