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| Status |
Public on May 23, 2023 |
| Title |
The identification of protein and RNA interactors of the splicing factor Caper in the adult Drosophila nervous system |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Post-transcriptional gene regulation is a fundamental mechanism that helps regulate the development and healthy aging of the nervous system. Mutations that disrupt the function of RNA-binding proteins (RBPs), which regulate post-transcriptional gene regulation, have increasingly been implicated in neurological disorders including amyotrophic lateral sclerosis, Fragile X Syndrome, and spinal muscular atrophy. Interestingly, although the majority of RBPs are expressed widely within diverse tissue types, the nervous system is often particularly sensitive to their dysfunction. It is therefore critical to elucidate how aberrant RNA regulation that results from the dysfunction of ubiquitously expressed RBPs leads to tissue specific pathologies that underlie neurological diseases. The highly conserved RBP and alternative splicing factor Caper is widely expressed throughout development and is required for the development of Drosophila sensory and motor neurons. Furthermore, caper dysfunction results in larval and adult locomotor deficits. Nonetheless, little is known about which proteins interact with Caper, and which RNAs are regulated by Caper. Here we identify proteins that interact with Caper in both neural and muscle tissue, along with neural specific Caper target RNAs. Furthermore, we show that a subset of these Caper-interacting proteins and RNAs genetically interact with caper to regulate Drosophila gravitaxis behavior.
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| Overall design |
RIPSeq experiment performed on Drosophila heads with FLAG-tagged Caper using either Caper or FLAG antibody.
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| Contributor(s) |
Titus MB, Chang AW, Popitsch N, Ebmeier CC, Bono JM, Olesnicky EC |
| Citation(s) |
37435576 |
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| Submission date |
Dec 20, 2022 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Michael Brandon Titus |
| E-mail(s) |
mtitus@uccs.edu
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| Organization name |
University of Colorado Colorado Springs
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| Lab |
Olesnicky
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| Street address |
1420 Austin Bluffs Pkwy Colorado Springs, CO USA 80918
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| City |
Colorado Springs |
| State/province |
Colorado |
| ZIP/Postal code |
80918 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA914215 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221381_feature_counts.tsv.gz |
1.1 Mb |
(ftp)(http) |
TSV |
| GSE221381_feature_counts.tsv.summary.txt.gz |
521 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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