|
| Status |
Public on Feb 22, 2024 |
| Title |
Maturation of Long Lived Plasma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To analyze global gene transcriptional changes and BCR clonal differences associated with long lived plasma cell specification, we sorted LLPCs and bulk PCs using Blimp1-ERT2-cre rosa26-LSLYFP expressing mice and analyzed them using bulk RNAsequencing
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| |
|
| Overall design |
on day 90 or day 5 after tamoxifen, YFP+ and YFP- CD138high B220- PCs were FACS-purified from BM, SPLEEN from young mice (age 6-8 for treatment) and middle-aged mice. (age 24 weeks at tamoxifen treatment), with 3-4 mice per group. Day 5 mice were aged 24 weeks before treatment
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| Web link |
https://doi.org/10.7554/eLife.89712.1
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| |
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| Contributor(s) |
Fooksman D, Jing Z, Zheng D, Galbo P, MacCarthy T |
| Citation(s) |
36891288 |
| |
| Submission date |
Dec 18, 2022 |
| Last update date |
Feb 22, 2024 |
| Contact name |
David Robert Fooksman |
| E-mail(s) |
david.fooksman@einsteinmed.edu
|
| Phone |
7188397957
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Pathology
|
| Lab |
Fooksman lab
|
| Street address |
1300 Morris Park
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (44)
|
|
| Relations |
| BioProject |
PRJNA913594 |
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