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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2023 |
| Title |
CRISPR deletion of ELAVL1 (HuR) in MIA PaCa-2 in vitro cells and in vivo tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the impact of the mRNA stabilty factor HuR on the pancreatic cancer transcriptome in MIA PaCa-2 in vitro cell line and in vivo tumors at multiple time points of tumor growth
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| Overall design |
CRISPR-mediated deletion of ELAVL1 (HuR) in MIA PaCa-2 PDAC cells. HuR wildtype (WT) or HuR knockout (KO) cells in culture (in vitro) were collected at 70% confuency for RNA extraction. HuR WT and HuR KO orthotopic tumors (in vivo) were harvested 2, 3, and 4 weeks post-injection for RNA extraction.
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| Contributor(s) |
McCarthy GA, Brody JR |
| Citation(s) |
37089813 |
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| Submission date |
Dec 18, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Grace Anne McCarthy |
| E-mail(s) |
mccarthg@ohsu.edu
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| Organization name |
Oregon Health & Science University
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| Department |
Department of Surgery
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| Street address |
2730 S Moody Ave
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| City |
Portland |
| State/province |
Oregon |
| ZIP/Postal code |
97201 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (27)
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| GSM6856845 |
HuR KO, Cell Line (KO_CL_4) |
| GSM6856846 |
HuR KO, Tumor, Week 2 (KO_Wk2_1) |
| GSM6856847 |
HuR KO, Tumor, Week 2 (KO_Wk2_3) |
| GSM6856848 |
HuR KO, Tumor, Week 3 (KO_Wk3_1) |
| GSM6856849 |
HuR KO, Tumor, Week 3 (KO_Wk3_2) |
| GSM6856850 |
HuR KO, Tumor, Week 3 (KO_Wk3_4) |
| GSM6856851 |
HuR KO, Tumor, Week 4 (KO_Wk4_1) |
| GSM6856852 |
HuR KO, Tumor, Week 4 (KO_Wk4_2) |
| GSM6856853 |
HuR KO, Tumor, Week 4 (KO_Wk4_3) |
| GSM6856854 |
HuR KO, Tumor, Week 4 (KO_Wk4_4) |
| GSM6856855 |
HuR WT, Cell Line (P_CL_1) |
| GSM6856856 |
HuR WT, Cell Line (P_CL_2) |
| GSM6856857 |
HuR WT, Cell Line (P_CL_3) |
| GSM6856858 |
HuR WT, Cell Line (P_CL_4) |
| GSM6856859 |
HuR WT, Tumor, Week 2 (P_Wk2_1) |
| GSM6856860 |
HuR WT, Tumor, Week 2 (P_Wk2_3) |
| GSM6856861 |
HuR WT, Tumor, Week 3 (P_Wk3_1) |
| GSM6856862 |
HuR WT, Tumor, Week 3 (P_Wk3_2) |
| GSM6856863 |
HuR WT, Tumor, Week 3 (P_Wk3_3) |
| GSM6856864 |
HuR WT, Tumor, Week 3 (P_Wk3_4) |
| GSM6856865 |
HuR WT, Tumor, Week 4 (P_Wk4_1) |
| GSM6856866 |
HuR WT, Tumor, Week 4 (P_Wk4_2) |
| GSM6856867 |
HuR WT, Tumor, Week 4 (P_Wk4_3) |
| GSM6856868 |
HuR WT, Tumor, Week 4 (P_Wk4_4) |
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| Relations |
| BioProject |
PRJNA913596 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221250_KO_CLvsP_CL_DEG.txt.gz |
3.2 Mb |
(ftp)(http) |
TXT |
| GSE221250_KO_Wk2vsP_Wk2_DEG.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
| GSE221250_KO_Wk3vsP_Wk3_DEG.txt.gz |
2.7 Mb |
(ftp)(http) |
TXT |
| GSE221250_KO_Wk4vsP_Wk4_DEG.txt.gz |
3.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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