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| Status |
Public on Feb 21, 2023 |
| Title |
Analysis of the role of Dectin-1 in the development of intestinal tumor-infiltrating myeloid cells [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the potential role of Dectin-1 in the development of intestinal tumor-infiltrating myeloid cells, we purified CD11b+ & CD11c+ cells from colorectal polyps of wild-type (WT) or Dectin-1 deficient (Clec7a–/–, KO) mice after azoxymethane (AOM) and 3 cycles of dextran-sodium-sulfate (DSS)-administration for 16 weeks, and compared the differences in the populations of each myeloid cell subset by scRNA-seq.
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| Overall design |
WT and KO mice were admnistrated with AOM (10mg/g) and 3 cycles of 1% DSS for 16 weeks. Colorectal polyps and non-polyp tissues were collected and CD11b+ & CD11c+ myeloid cells were purified by BD FACS Fusion. These cells were then applied for single cell RNA-sequencing.
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| Contributor(s) |
Tang C |
| Citation missing |
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| Submission date |
Dec 16, 2022 |
| Last update date |
Feb 23, 2023 |
| Contact name |
Ce Tang |
| E-mail(s) |
tangc7@mail.sysu.edu.cn
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| Phone |
+86-13693417116
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| Organization name |
The First Affiliated Hospital, Sun Yat-sen University
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| Street address |
No.58, Zhong Shan Er Lu
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| City |
Guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE221206 |
Analysis of the role of Dectin-1 in the development of intestinal tumor-infiltrating myeloid cells and intestinal tumorigenesis |
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| Relations |
| BioProject |
PRJNA913187 |
