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| Status |
Public on Oct 18, 2010 |
| Title |
Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization to enable precise gene regulation: MNase-seq data |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Metazoan transcription is controlled through either coordinated recruitment of transcription machinery to the gene promoter, or subsequently, through regulated pausing of RNA polymerase II (Pol II) in early elongation. We report that a key difference between genes that use these distinct regulatory strategies lies in the chromatin architecture specified by their DNA sequences. Pol II pausing is prominent at highly-regulated genes whose sequences inherently disfavor nucleosome formation within the gene, but favor nucleosomal occlusion of the promoter. Pausing of polymerase maintains these genes in an active state by inhibiting the formation of repressive promoter chromatin. In contrast, promoters of housekeeping genes that lack paused Pol II are deprived of nucleosomes regardless of polymerase binding, but show higher nucleosome occupancy downstream. Our results suggest that the "default" chromatin state of a gene instructs its regulation, and that highly-regulated promoters have evolved to encourage competition between nucleosomes and paused Pol II for promoter occupancy.
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| Overall design |
Examination of MNase-digested mononucleosomal chromatin from untreated, mock-treated, and NELF-depleted Drosophila S2 cells.
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| Contributor(s) |
Gilchrist DA, Dos Santos G, Gao Y, Li L, Fargo DC, Adelman K |
| Citation(s) |
21074046 |
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| Submission date |
Jun 03, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Karen Adelman |
| E-mail(s) |
karen_adelman@hms.harvard.edu
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| Organization name |
Harvard Medical School
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| Department |
Biological Chemistry and Molecular Pharmacology
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| Street address |
45 Shattuck St. LHRRB-201a
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL9061 |
Illumina Genome Analyzer II (Drosophila melanogaster) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE20472 |
Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization to enable precise gene regulation |
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| Relations |
| SRA |
SRP002568 |
| BioProject |
PRJNA127605 |
