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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 02, 2023 |
| Title |
Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation [scRNASeq] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ability of the pluripotent epiblast to contribute progeny to all three germ layers is thought to be lost after gastrulation. The later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential to generate mesodermal- and endodermal-like cells is obtained. We monitored transcriptional changes from gastrulation to neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. Here we show maintenance of pluripotency-signature (Nanog/Oct4-PouV/Klf4-positive) in undecided pan-ectodermal stem-cells spanning the entire ectoderm late during neurulation with ectodermal patterning completed only at the end of neurulation when the pluripotency-signature becomes restricted to NC, challenging our understanding of gastrulation. Furthermore, broad ectodermal pluripotency-signature is found at all axial levels unrelated to the NC lineage the cells later commit to, suggesting a general role in stemness enhancement and proposing a mechanism by which the NC acquires its ability to form derivatives beyond “ectodermal-capacity” in chick and mouse embryos.
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| Overall design |
Chick embryo samples from all tissue at the midbrain axial level from HH5, 1ss, 4ss and 7ss stage were dissociated and analyzed by scRNAseq. SEURAT workflow for scRNAseq analysis was followed by differential expression between ectodermal cell types.
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| Contributor(s) |
Pajanoja C, Hsin J, Olinger B, Schiffmacher A, Yazejian R, Abrams S, Dapkunas A, Zainul Z, Doyle A, Martin D, Kerosuo L |
| Citation(s) |
36747797 |
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| Submission date |
Dec 16, 2022 |
| Last update date |
May 17, 2023 |
| Contact name |
Laura Kerosuo-Pahlberg |
| E-mail(s) |
laura.kerosuopahlberg@nih.gov
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| Organization name |
NIDCR
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| Street address |
30 Convent Dr
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (8)
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| GSM6850267 |
1ss, replicate 2, scRNAseq |
| GSM6850268 |
4ss, replicate 1, scRNAseq |
| GSM6850269 |
4ss, replicate 2, scRNAseq |
| GSM6850270 |
7ss, replicate 1, scRNAseq |
| GSM6850271 |
7ss, replicate 2, scRNAseq |
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| This SubSeries is part of SuperSeries: |
| GSE221190 |
Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation |
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| Relations |
| BioProject |
PRJNA913169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221188_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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