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| Status |
Public on Dec 14, 2023 |
| Title |
Determination of Shavenbaby target genes in mature Enterocytes (mECs) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Shavenbaby (Svb) transcription factor is involved in the homeostasis of intestinal stem cells (ISCs), and the differentiation of their enteroblast progeny (EB) into enterocytes (EC). Svb is produced as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides SvbREP is processed into a shorter activator form (SvbACT) through partial proteasomal degradation of the REP domain.
In ISCs/EBs, Pri triggers SvbREP to ACT maturation, while SvbREP form accumulates in differentiated enterocytes. The absence of Svb promote the apoptosis of the intestinal stem cells. In ISCs/EBs, the ectopic expression of SvbREP form promotes the differentiation and the overexpression of SvbACT promotes hyperplasia. (Al Hayek, et al 2020)
Based on these functional analyses, Svb appears to control ISC/EB survival and behavior, balancing proliferation and differentiation through a molecular switch between its 2 antagonistic isoforms. In this study, we determine the Svb target genes in different cell types along the intestinal cell lineage.
This particular metadata sheet refers to early enterocytes (mEC) transcriptomic data (RNAseq) in control condition and upon modulation of Svb function.
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| Overall design |
The samples correspond to control (mEC Ctrl), Svb-knock down ECs (mEC RNAiSvb), SvbACT constitutive form overexpression (mEC SvbAct). Three biological replicates were performed for each genotype (except for the Ctrl n=2), on different calendar days.
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| Contributor(s) |
Mancheno-Ferris A, Immarigeon C |
| Citation(s) |
38174321 |
| |
| Submission date |
Dec 08, 2022 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Cedric Polesello |
| Organization name |
Centre de Biologie Intégrative de Toulouse
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| Street address |
118 Route de Narbonne
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| City |
Toulouse |
| ZIP/Postal code |
310362 |
| Country |
France |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE220561 |
Determination of Shavenbaby target genes in Intestinal Stem Cells differenciation |
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| Relations |
| BioProject |
PRJNA910222 |
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