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| Status |
Public on May 29, 2010 |
| Title |
Identification of genes that regulated by IRF4 in human cutaneous T cell lymphoma HuT-102 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The microarray analysis showed an interesting up-regulation in the set of genes controlling the development of Th1, mainly IFN-gamma, and other type 1 interferon response genes including CXCL10 in IRF4 knocked-down HuT-102 cells.
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| Overall design |
HuT-102 cells, a human cutaneous T cell lymphoma, were transiently transfected with siRNAs against human IRF4 or firefly luciferase. Cells were incubated for 60 h at 37 centigrade. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturer’s instructions.
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| Contributor(s) |
Refaat A, Zhou Y, Tabuchi Y, Takasaki I, Sakurai H |
| Citation(s) |
21498517 |
| |
| Submission date |
May 27, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
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| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Lab |
Division of Molecular Genetics Research
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| GSM547811 |
Control-1 (HuT-102 cells + luciferase siRNA) |
| GSM547812 |
Control-2 (HuT-102 cells + luciferase siRNA) |
| GSM547813 |
siRNA-IRF4-1 (HuT-102 cells + IRF4 siRNA) |
| GSM547814 |
siRNA-IRF4-2 (HuT-102 cells + IRF4 siRNA) |
|
| Relations |
| BioProject |
PRJNA128883 |
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