 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 29, 2023 |
| Title |
Spt3 and Spt8 are involved in the formation of a silencing boundary by interacting with TATA-binding protein |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
In Saccharomyces cerevisiae, a heterochromatin-like chromatin structure called the silencing region is present at the telomere as a complex of Sir2, Sir3, and Sir4. Sir2 is a histone deacetylase, and Sir3 and Sir4 promote spreading of the silencing region at the telomere. Histone acetyltransferases restrict the silencing region. Here, we show that Spt3 and Spt8 block the spread of silencing regions. Spt3 and Spt8 are members of the Spt-Ada-Gcn5-acetyltransferase (SAGA) complex, which has histone acetyltransferase activity. Although both Spt3 and Spt8 interact with telomere-binding proteins (TBPs), Spt3 had a greater effect on genome-wide transcription. Mutant analysis showed that TBPs play an important role in boundary formation, in particular the interaction between Spt3 and TBPs.
|
| |
|
| Overall design |
All strain cells were grown at 30ºC in 5ml YPD media with shaking. Total RNAs were collected at exponential phase (optimal density of 0.7) using acid phenol-chloroform extraction method and hybridized on Affymetrix microarrays.
|
| |
|
| Contributor(s) |
Kamata K, Ayano T, Oki M |
| Citation(s) |
37189367 |
| |
| Submission date |
Dec 07, 2022 |
| Last update date |
Jun 28, 2023 |
| Contact name |
Takahito Ayano |
| E-mail(s) |
cb.0000g.c.t@gmail.com
|
| Organization name |
University of Fukui
|
| Department |
Graduate school engineering
|
| Street address |
3-9-1, Bunkyo
|
| City |
Fukui-city |
| State/province |
Fukui |
| ZIP/Postal code |
910-8507 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA909582 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220290_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
