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| Status |
Public on Jul 01, 2010 |
| Title |
FnrL regulon in Rhodobacter sphaeroides |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
In this study, we performed a ChIP-chip experiment to determine the regulon of FnrL in Rhodobacter sphaeroides. We grew R. sphaeroides under anaerobic photosnthetic conditions, in which FnrL is expected to bind DNA and contol gene expression, and immuno-precipitated FnrL, but also sigma70 and the Beta' subunits of RNA polymerase to determine transcription activity.
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| Overall design |
DNA immunoprecipitated with polyclonal antibodies against FnrL, sigma70, or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.
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| |
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| Contributor(s) |
Dufour YS, Donohue TJ, Kiley PJ |
| Citation(s) |
20661434 |
| |
| Submission date |
May 27, 2010 |
| Last update date |
Jul 29, 2012 |
| Contact name |
Yann S Dufour |
| Organization name |
University of Wisconsin - Madison
|
| Department |
Bacteriology
|
| Lab |
Timothy Donohue
|
| Street address |
1550 Linden Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL10463 |
UW_Rhodobacter_sphaeroides_2.4.1_385K_v2 |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA127453 |
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