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Series GSE22027 Query DataSets for GSE22027
Status Public on Jul 01, 2010
Title FnrL regulon in Rhodobacter sphaeroides
Organism Cereibacter sphaeroides 2.4.1
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary In this study, we performed a ChIP-chip experiment to determine the regulon of FnrL in Rhodobacter sphaeroides. We grew R. sphaeroides under anaerobic photosnthetic conditions, in which FnrL is expected to bind DNA and contol gene expression, and immuno-precipitated FnrL, but also sigma70 and the Beta' subunits of RNA polymerase to determine transcription activity.
 
Overall design DNA immunoprecipitated with polyclonal antibodies against FnrL, sigma70, or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.
 
Contributor(s) Dufour YS, Donohue TJ, Kiley PJ
Citation(s) 20661434
Submission date May 27, 2010
Last update date Jul 29, 2012
Contact name Yann S Dufour
Organization name University of Wisconsin - Madison
Department Bacteriology
Lab Timothy Donohue
Street address 1550 Linden Drive
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL10463 UW_Rhodobacter_sphaeroides_2.4.1_385K_v2
Samples (9)
GSM547633 FnrL_rep 1
GSM547634 FnrL_rep 2
GSM547635 FnrL_rep 3
Relations
BioProject PRJNA127453

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22027_RAW.tar 178.9 Mb (http)(custom) TAR (of GFF, PAIR)
Processed data included within Sample table
Processed data provided as supplementary file

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