|
| Status |
Public on Dec 10, 2022 |
| Title |
Discovery and Functional Analysis of Secondary Hair Follicle miRNAs during Annual Cashmere Growth |
| Organism |
Capra hircus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Secondary hair follicles (SHF) produce the thermoregulatory cashmere in goat. MiRNAs were reported playing indispensable roles in hair follicle formation and growth. However, most studies examining miRNAs related to cashmere have been performed on goat skin. It still remains obscure that which miRNAs are highly expressed in SHFs or how miRNAs affect the cashmere growth. In the present study, we isolated the SHFs under dissecting microscope and analyzed the miRNA signatures during annual cashmere growth. Small RNA sequencing followed by genome-wide expression analysis reveals that early anagen is a crucial phase for miRNA regulation on the cashmere growth, which was uncovered by two predominant groups of miRNAs. Although they exhibit opposite expression patterns, both groups demonstrated sharp changes of expression when transit from early anagen to mid-anagen. In addition, we identified 96 miRNA signatures that differentially expressed between different phases among 376 miRNAs. Functional analysis of the predicted target genes for highly expressed or differentially expressed miRNAs indicated that these miRNAs were involved in signal pathways associated with SHF development, regeneration and regression. Furthermore, miR-143-3p is preferentially expressed in SHFs and Itga6 was identified as one of targets. The dual-luciferase and in situ hybridization assay demonstrated that miR-143-3p directly repressed the expression of Itga6, suggesting a possible novel role for miR-143-3p in the cashmere growth.
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| Overall design |
Genome-wide discovery and molecular characterization of secondary hair follicle miRNAs in cashmere goat across six-time points each with two biological replicates (male and female), representing phases of anagen (May and Sep), catagen (Nov and Dec) and telogen (Feb and Mar).
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| Contributor(s) |
Xue H, Wang M, Dai H, Sheng S, Liu Y |
| Citation(s) |
36674578 |
| |
| Submission date |
Dec 06, 2022 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Wang Ming Lin |
| E-mail(s) |
amazinglin@126.com
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| Organization name |
shenyang agricultural university
|
| Street address |
120 Dongling Road
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| City |
Shenyang |
| ZIP/Postal code |
110866 |
| Country |
China |
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| Platforms (1) |
| GPL19149 |
Illumina HiSeq 2500 (Capra hircus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA909238 |
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