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| Status |
Public on Sep 04, 2023 |
| Title |
Effect of high-dose glucocorticoid treatment on human brown adipose tissue activity: a randomised, double-blinded, placebo-controlled cross-over trial in healthy men |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Glucocorticoids (GCs) are widely applied anti-inflammatory drugs that are associated with adverse metabolic effects including insulin resistance and weight gain. Previous research indicated that GCs may negatively impact brown adipose tissue (BAT) in rodents and humans. Here, we demonstrate that treatment of human volunteers with high doses of GCs increases resting energy expenditure (REE) but does not reduce BAT activity. In line with the physiological results, gene expression in human BAT was not significantly altered by GCs in vivo. However, the RNA-sequencing of skeletal muscle revealed that GCs increase the expression of enzymes involved in calcium cycling as well as the respiratory chain. Collectively, these data suggest that the raise of EE after GCs exposure is mediated by skeletal muscle rather than a change in BAT activity.
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| Overall design |
Randomized crossover trial
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| |
|
| Contributor(s) |
Maushart CI, Ghosh A, Sun W, Wolfrum C, Betz MJ |
| Citation(s) |
37659283 |
| |
| Submission date |
Dec 05, 2022 |
| Last update date |
Sep 05, 2023 |
| Contact name |
Adhideb Ghosh |
| E-mail(s) |
adhideb.ghosh@hest.ethz.ch
|
| Organization name |
ETH Zurich
|
| Department |
Health Sciences and Technology
|
| Lab |
Translational Nutrition Biology
|
| Street address |
Schorenstrasse 16
|
| City |
Schwerzenbach |
| ZIP/Postal code |
8603 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (53)
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| Relations |
| BioProject |
PRJNA908886 |
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