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| Status |
Public on May 20, 2010 |
| Title |
Propagation of Adipogenic Signals through an Epigenomic Transition State |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), p300, mediator subunit 1, and histone H3 acetylation near genes involved in cell proliferation, development and differentiation, including the gene encoding the master regulator of adipocyte differentiation, peroxisome proliferator activated receptor g2 (PPARg2). Occupancy and enhancer function are triggered by adipogenic signals, and diminish upon their removal. GR, which is required for adipogenesis but need not be active in the mature adipocyte, transiently functions with other enhancer proteins to propagate a new program of gene expression that includes induction of PPARg2, thereby providing a memory of the earlier adipogenic signal. Thus, the conversion of preadipocyte to adipocytes involves the formation of an epigenomic transition state that is not observed in cells at the beginning or end of the differentiation process.
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| Overall design |
Genomic occupancy profiled by high throughput sequencing (ChIP-seq) from 3T3-L1 cells during differentiation for H3K9Ac, CEBPb and GR.
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| Contributor(s) |
Steger DJ, Lazar MA |
| Citation(s) |
20478996 |
| Submission date |
May 19, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
David Steger |
| E-mail(s) |
stegerdj@mail.med.upenn.edu
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| Organization name |
University of Pennsylvania
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| Street address |
415 Curie Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (10)
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| Relations |
| SRA |
SRP002507 |
| BioProject |
PRJNA126859 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21898_3T3-L1_input_bias.txt.gz |
139 b |
(ftp)(http) |
TXT |
| GSE21898_RAW.tar |
550.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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