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| Status |
Public on Aug 14, 2023 |
| Title |
Brain transcriptome of mice infected with T. gondii parasites |
| Organisms |
Toxoplasma gondii; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mice were infected with up to 250,000 tachyzoites of T. gondii VEG strain parasites genetically engineered to be deficient in either the ROCY1 gene (TGVEG_311100) or the BFD1 gene (TGVEG_200385). Mice were infected intraperitoneally and monitored using in vivo bioluminescence imaging. Mice were sacrificed at either 4 weeks or 9 weeks post infection as indicated, and RNA was isolated from their brains and transcript abundance was determined using RNAseq.
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| Overall design |
4-6 biological replicates for the 4 week samples and only 1-2 replicates for the 9 weeks samples.
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| Contributor(s) |
Borrelli SS, Cabo LF, Boyle JP |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI114655 |
Effector diversification and Toxoplasma virulence |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Jon P Boyle |
| R01 AI116855 |
Comparative and functional genomics of Toxoplasma and Hammondia hammondi |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Jon P Boyle |
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| |
| Submission date |
Nov 28, 2022 |
| Last update date |
Aug 14, 2023 |
| Contact name |
Jon P Boyle |
| E-mail(s) |
boylej@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Biological Sciences
|
| Street address |
4249 Fifth Avenue
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15260 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL32893 |
Illumina NextSeq 500 (Mus musculus; Toxoplasma gondii) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA906133 |
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