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| Status |
Public on Sep 12, 2024 |
| Title |
MrgprA3 neurons selectively control myeloid-derived IL-33 for IL-17 dependent cutaneous immunity [bulk RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skin contains poorly understood interdependent cellular networks that facilitate barrier integrity and host immunity, including neurons, and myeloid cells; the latter of which intrinsically express the pleiotropic cytokine IL-33. This work shows selective suppression of IL-33 expression in myeloid cells by activation of itch-sensing neurons bearing the Mas-related G protein receptor A3 (A3). Optogenetic activation of A3 neurons also increased IL-17-expressing γδ T cells, epidermal thickening, and resistance to the human pathogen Schistosoma mansoni, partially through the neuropeptide CGRP. Cell-intrinsic loss of IL-33 in myeloid cells alters chromatin conformation, basally elevates expression and release of IL-17-inducing cytokines (e.g., IL-1β, IL-6), and expands macrophage and cDC2 differentiation, driving both tissue pathology (e.g., epidermal thickening, keratinocyte hyperplasia) and resistance to helminth infection. Our findings suggest a mechanism of cellular cross-talk allowing “itch” neuron activation to alter myeloid composition and cytokine expression patterns for reshaping skin architecture and driving immunity
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| Overall design |
3 abdominal skin biopsies from naïve CD11c control or CD11c-specific IL-33KO mice
Raw data not provided for GSM6757194 and GSM6757197
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| Contributor(s) |
Inclan-Rico JM, Napuri C, Hung L, Pastore CF, Lin C, Ferguson A, Rossi HL, Kambayashi T, Reed D, Herbert DR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 27, 2022 |
| Last update date |
Sep 12, 2024 |
| Contact name |
Juan M. Inclan-Rico |
| E-mail(s) |
jinclan@upenn.edu
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| Organization name |
University of Pennsylvania, School of Veterinary Medicine
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| Department |
Pathobiology
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| Street address |
3800 Spruce St
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE218834 |
MrgprA3 neurons selectively control myeloid-derived IL-33 for IL-17 dependent cutaneous immunity |
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| Relations |
| BioProject |
PRJNA905814 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE218826_Kallisto_counts.tsv.gz |
782.8 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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