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| Status |
Public on Feb 06, 2023 |
| Title |
Gene expression analysis of THP-1 cells (monocytic cell line) transfected with microRNA mimics: let-7d-3p, let-7e-5p,miR-146a-5p,miR-130a-3p, miR-151a-3p. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In our previous study, hsa-let-7d-3p, hsa-let-7e-5p,hsa-miR-146a-5p,hsa-miR-130a-3p, hsa-miR-151a-3p,were significantly upregulated in the plasma of atopic patients.
To study the each function of let-7d-3p, let-7e-5p,miR-146a-5p,miR-130a-3p, miR-151a-3p which are significantly upregulated in the plasma of atopic patients, we performed mimic-transfected THP-1 cells, a mononuclear cell line, and performed comprehensive genetic analysis.
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| Overall design |
THP-1 cells were transfected with let-7d-3p, let-7e-5p,miR-146a-5p,miR-130a-3p, miR-151a-3p mimics or negative controls, respectively, and RNA was extracted. This RNA was then used for hybridization on Affymetrix microarrays.
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| Contributor(s) |
Nishigaki H, Ueta M |
| Citation(s) |
36685889 |
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| Submission date |
Nov 14, 2022 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Hiromi Nishigaki |
| E-mail(s) |
ngaki-hi@koto.kpu-m.ac.jp
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| Organization name |
Kyoto Prefectural University of Medicine
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| Street address |
465 Kajii-Cyo, Hirokouji, Kawaramachi doori, Kamigyo-ku
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| City |
Kyoto City |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA901496 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE217971_RAW.tar |
6.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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