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| Status |
Public on May 11, 2010 |
| Title |
Expression data from Arabidopsis gapcp mutant treated with ABA |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2). gapcp double mutants (gapcp1 gapcp2) display a drastic phenotype of arrested root development and sterility.Complex interactions occurring between ABA and sugar signal transduction pathways have been shown, but the molecular mechanisms connecting both pathways are not well understood. Since we found drastic carbohydrate changes in gapcp1 gapcp2, we studied their response to ABA. by performing a microarray analysis comparing gapcp1 gapcp2 and wild type seedlings after a long term treatment with ABA.
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| Overall design |
15-day old Arabidopsis seedlings (Col 0) and gapcp1gapcp2 double mutants were used for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Ros R |
| Citation(s) |
21068209 |
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| Submission date |
May 10, 2010 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Roc Ros |
| E-mail(s) |
roc.ros@uv.es
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| Organization name |
Universitat de Valencia
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| Department |
Biologia Vegetal
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| Street address |
Vicent Andres Estelles
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| City |
Burjassot |
| State/province |
valencia |
| ZIP/Postal code |
46100 |
| Country |
Spain |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM542489 |
wild type with ABA, biological rep1 |
| GSM542490 |
wild type with ABA, biological rep2 |
| GSM542491 |
wild type with ABA, biological rep3 |
| GSM542492 |
gapcp1gapcp2 with ABA, biological rep1 |
| GSM542493 |
gapcp1gapcp2 with ABA, biological rep2 |
| GSM542494 |
gapcp1gapcp2 with ABA, biological rep3 |
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| Relations |
| BioProject |
PRJNA127283 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21765_RAW.tar |
19.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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