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| Status |
Public on Mar 22, 2023 |
| Title |
Chimeric EGFR ChIP-seq of an MCF-10A clone expressing chimeric EGFR and Erbb2 receptors |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Synthetic activation of chimeric EGFR-Erbb2 receptors causes nuclear translocation of the heterodimers at long time scales. To identify genomic loci bound by nuclear EGFR-Erbb2, chromatin from an MCF10A-5E clone coexpressing chimeric EGFR and Erbb2 was prepared with or without synthetic activation and immunoprecipitated for chimeric EGFR by its GluGlu epitope tag.
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| Overall design |
The EGFR-Erbb2 MCF10A-5E clone (B2B1) was grown in overlay culture for six days, stimulated with or without 0.5 µM AP21967 for 24 hours, and prepared for chromatin immunoprecipitation with GluGlu antibody (RRID:AB_2191052) or naïve rabbit IgG as described (Wang et al., Proc. Natl. Acad. Sci. 108:E803-12 [2011]).
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| Contributor(s) |
Paudel BB, Janes KA |
| Citation(s) |
37055441 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U54 CA274499 |
Systems Analysis of Stress-adapted Cancer Organelles (SASCO) Center |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Kevin A Janes |
| U01 CA215794 |
An Integrated Systems Approach for Incompletely Penetrant Onco-phenotypes. |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Kevin A Janes |
| R50 CA265089 |
Definition and perturbation of cell-regulatory heterogeneities in solid tumors |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Lixin Wang |
| P30 CA044579 |
University of Virginia Cancer Center Support Grant |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Thomas Patrick Loughran |
| P30 CA054174 |
Mays Cancer Center at UT Health SA |
UNIVERSITY OF TEXAS HLTH SCI CTR SAN ANTONIO |
Patrick Sung |
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| |
| Submission date |
Oct 20, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Kevin A. Janes |
| E-mail(s) |
kjanes@virginia.edu
|
| Organization name |
University of Virginia
|
| Department |
Department of Biomedical Engineering
|
| Street address |
Box 800759 Health System
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA892637 |
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