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Status |
Public on Mar 22, 2023 |
Title |
Chimeric EGFR ChIP-seq of an MCF-10A clone expressing chimeric EGFR and Erbb2 receptors |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Synthetic activation of chimeric EGFR-Erbb2 receptors causes nuclear translocation of the heterodimers at long time scales. To identify genomic loci bound by nuclear EGFR-Erbb2, chromatin from an MCF10A-5E clone coexpressing chimeric EGFR and Erbb2 was prepared with or without synthetic activation and immunoprecipitated for chimeric EGFR by its GluGlu epitope tag.
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Overall design |
The EGFR-Erbb2 MCF10A-5E clone (B2B1) was grown in overlay culture for six days, stimulated with or without 0.5 µM AP21967 for 24 hours, and prepared for chromatin immunoprecipitation with GluGlu antibody (RRID:AB_2191052) or naïve rabbit IgG as described (Wang et al., Proc. Natl. Acad. Sci. 108:E803-12 [2011]).
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Contributor(s) |
Paudel BB, Janes KA |
Citation(s) |
37055441 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
U54 CA274499 |
Systems Analysis of Stress-adapted Cancer Organelles (SASCO) Center |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Kevin A Janes |
U01 CA215794 |
An Integrated Systems Approach for Incompletely Penetrant Onco-phenotypes. |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Kevin A Janes |
R50 CA265089 |
Definition and perturbation of cell-regulatory heterogeneities in solid tumors |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Lixin Wang |
P30 CA044579 |
University of Virginia Cancer Center Support Grant |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Thomas Patrick Loughran |
P30 CA054174 |
Mays Cancer Center at UT Health SA |
UNIVERSITY OF TEXAS HLTH SCI CTR SAN ANTONIO |
Patrick Sung |
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Submission date |
Oct 20, 2022 |
Last update date |
May 03, 2023 |
Contact name |
Kevin A. Janes |
E-mail(s) |
kjanes@virginia.edu
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Organization name |
University of Virginia
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Department |
Department of Biomedical Engineering
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Street address |
Box 800759 Health System
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City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (12)
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Relations |
BioProject |
PRJNA892637 |