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| Status |
Public on Aug 03, 2023 |
| Title |
Decoding the endometrial niche of Asherman's syndrome at single-cell resolution |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Asherman’s Syndrome is characterized by intrauterine adhesions or scarring, which cause infertility, menstrual abnormalities, and recurrent pregnancy loss. The pathophysiology of this syndrome remains unknown, with treatment restricted to recurrent surgical removal of intrauterine scarring, which has limited success. Here, we decode the Asherman’s Syndrome endometrial cell niche by analyzing data from over 200,000 cells with single-cell RNA-sequencing in patients with this condition and through in vitro analyses of Asherman’s Syndrome patient-derived endometrial organoids. Our endometrial atlas highlights the loss of the endometrial epithelium, alterations to epithelial differentiation signaling pathways such as Wnt and Notch, and the appearance of characteristic epithelium expressing secretory leukocyte protease inhibitor during the window of implantation. We describe syndrome-associated alterations in cell-to-cell communication and gene expression profiles that support a dysfunctional pro-fibrotic, pro-inflammatory, and anti-angiogenic environment.
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| Overall design |
We have samples from Asherman patients in pre-treatment and WOI control samples.
***Submitter declares that the raw data files will be raw data will be deposited in dbGaP as information involves living donors under GDPR restrictions related to clinical trials (https://www.clinicaltrialsregister.eu/ctr-search/trial/2016-003975-23/ES) with signed informed consent. ***
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| Web link |
https://www.nature.com/articles/s41467-023-41656-1
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| Contributor(s) |
Santamaria X, Roson B, Perez-Moraga R, Venkatesan N, Pardo-Figuerez M, Gonzalez-Fernandez J, Llera-Oyola J, Fernández E, Moreno I, Salumets A, Vankelecom H, Vilella F, Simon C |
| Citation(s) |
37735465 |
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| Submission date |
Oct 17, 2022 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Raul Pérez-Moraga |
| E-mail(s) |
rperez@fundacioncarlossimon.com
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| Organization name |
Carlos Simon Foundation, INCLIVA Health Research Institute
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| Street address |
Rda. de Narcís Monturiol, 11, Bloque C
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| City |
Paterna |
| State/province |
Valencia |
| ZIP/Postal code |
46980 |
| Country |
Spain |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA891357 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215968_RAW.tar |
2.8 Gb |
(http)(custom) |
TAR (of TAR) |
| GSE215968_sc_AS_pre_vs_post.h5ad.gz |
1.6 Gb |
(ftp)(http) |
H5AD |
| GSE215968_sc_AS_vs_WOI_Control.h5ad.gz |
1.3 Gb |
(ftp)(http) |
H5AD |
| GSE215968_sc_CD133.h5ad.gz |
642.3 Mb |
(ftp)(http) |
H5AD |
| Processed data are available on Series record |
| Raw data not provided for this record |
| Processed data provided as supplementary file |
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