|
| Status |
Public on May 05, 2010 |
| Title |
Interactions between Streptococcus thermophilus and Lactobacillus bulgaricus in yoghurt |
| Organisms |
Streptococcus thermophilus CNRZ1066; Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 |
| Experiment type |
Expression profiling by array
|
| Summary |
Many food fermentations are carried out by mixed cultures of lactic acid bacteria. Interactions between strains are of key importance for the performance of these fermentations. Yoghurt fermentation by Streptoccus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus (L.bulgaricus) is one of the best-described mixed culture fermentations. These species stimulate each other’s growth by the exchange of metabolites such as folic acid and carbon dioxide. Recently, post-genomic studies have been applied to reveal the global physiological response to mixed culture growth in S. thermophilus, but an in-depth molecular analysis of mixed culture growth of both strains remains to be established. Here we report the application of mixed culture transcriptome profiling and a systematic analysis of candidate interaction compounds on growth, which allowed the unraveling of the molecular responses associated with co-culture growth in batch of S. thermophilus CNRZ1066 and L. bulgaricus ATCC BAA-365 in milk.
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| Overall design |
Comparisons of mono cultures versus mixed cultures, at four time-points in batch fermentation, and comparisons between the four time-points within each fermentation, all in duplicate
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| Contributor(s) |
Sieuwerts S, Molenaar D, van Hijum SA, Beerthuyzen M, Stevens MJ, Janssen PW, de Bok FA, de Vos WM, van Hylckama Vlieg JE |
| Citation(s) |
20889781 |
| |
| Submission date |
Apr 29, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Sander Sieuwerts |
| Organization name |
NIZO Food Research
|
| Department |
Health
|
| Street address |
Kernhemseweg 2
|
| City |
Ede |
| ZIP/Postal code |
6718ZB |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL10366 |
WCFS yoghurt arrays, TB1_8, Version 1 |
|
| Samples (20)
|
| GSM539046 |
Mono cultures 5.5 h rep1 _ Mono cultures 3.5 h rep1 |
| GSM539047 |
Mono cultures 8 h rep1 _ Mono cultures 5.5 h rep1 |
| GSM539048 |
Mono cultures 12 h rep1 _ Mono cultures 8 h rep1 |
| GSM539049 |
Mixed culture 3.5 h rep1 _ Mixed culture 5.5 h rep1 |
| GSM539050 |
Mixed culture 5.5 h rep1 _ Mixed culture 8 h rep1 |
| GSM539051 |
Mixed culture 8 h rep1 _ Mixed culture 12 h rep1 |
| GSM539052 |
Mono cultures 3.5 h rep2 _ Mono cultures 5.5 h rep2 |
| GSM539053 |
Mono cultures 5.5 h rep2 _ Mono cultures 8 h rep2 |
| GSM539054 |
Mono cultures 8 h rep2 _ Mono cultures 12 h rep2 |
| GSM539055 |
Mixed culture 5.5 h rep2 _ Mixed culture 3.5 h rep2 |
| GSM539056 |
Mixed culture 8 h rep2 _ Mixed culture 5.5 h rep2 |
| GSM539057 |
Mixed culture 12 h rep2 _ Mixed culture 8 h rep2 |
| GSM539058 |
Mono cultures 3.5 h rep1 _ Mixed culture 3.5 h rep1 |
| GSM539059 |
Mixed culture 5.5 h rep1 _ Mono cultures 5.5 h rep1 |
| GSM539060 |
Mono cultures 8 h rep1 _ Mixed culture 8 h rep1 |
| GSM539061 |
Mixed culture 12 h rep1 _ Mono cultures 12 h rep1 |
| GSM539062 |
Mixed culture 3.5 h _ Mono cultures 3.5 h (3 merged hybs) |
| GSM539063 |
Mono cultures 5.5 h rep2 _ Mixed culture 5.5 h rep2 |
| GSM539064 |
Mixed culture 8 h rep2 _ Mono cultures 8 h rep2 |
| GSM539065 |
Mono cultures 12 h _ Mixed culture 12 h (3 merged hybs) |
|
| Relations |
| BioProject |
PRJNA127125 |
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