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| Status |
Public on Mar 31, 2023 |
| Title |
Fate restricted stromal fibroblasts and adipocytes demonstrate multi-modal responses to tissue injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adult connective tissues rearrange themselves during injury, disease, and aging. This response is widely considered to involve cross-lineage conversion between two differentiated stromal cell types: fibroblasts and adipocytes. Here, using lineage tracing approach, we explore the transcriptome profiles of Adipoq-lineage positve mature adipocytes and En1-lineage postive fibroblasts in in vivo mouse skin wounds. The data presented here show that wound adipocytes and fibroblasts still possess distinct molecular signatures, and do not undergo cross-lineage conversion.
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| Overall design |
Wound adipocytes and fibroblasts were FACS sorted as GFP+ cells from day 10 and day 21 wounds from AdipoqCre;R26mTmG and En1Cre;R26mTmG mice. Sorted cells were subjected to RNA-sequencing. Comparative gene expression profiling analysis of RNA-seq data of wound adipocytes and fibroblasts.
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| Contributor(s) |
Jiang D, Zhao J, Dai R, Lin Y, Rinkevich Y |
| Citation(s) |
37230982 |
| BioProject |
PRJNA889718 |
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| Submission date |
Oct 17, 2022 |
| Last update date |
Jul 01, 2023 |
| Contact name |
Dongsheng Jiang |
| Organization name |
Helmholtz Zentrum München
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| Department |
Institute of Regenerative Biology and Medicine
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| Street address |
Max-Lebsche-Platz 31
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM6646700 |
AD, d10, Replicate 1 |
| GSM6646701 |
AD, d10, Replicate 2 |
| GSM6646702 |
AD, d10, Replicate 3 |
| GSM6646703 |
AD, d21, Replicate 1 |
| GSM6646704 |
AD, d21, Replicate 2 |
| GSM6646706 |
AD, d21, Replicate 3 |
| GSM6646707 |
FB, NS, Replicate 1 |
| GSM6646708 |
FB, NS, Replicate 2 |
| GSM6646709 |
FB, NS, Replicate 3 |
| GSM6646710 |
FB, d10, Replicate 1 |
| GSM6646712 |
FB, d10, Replicate 2 |
| GSM6646713 |
FB, d10, Replicate 3 |
| GSM6646714 |
FB, d21, Replicate 1 |
| GSM6646715 |
FB, d21, Replicate 2 |
| GSM6646716 |
FB, d21, Replicate 3 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE215912_readCount_geneid.tsv.gz |
672.9 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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