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| Status |
Public on Jun 25, 2010 |
| Title |
Immature cell populations and an erythropoiesis gene expression signature in systemic juvenile idiopathic arthritis: Implications for pathogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Objective. Previous observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene expression signature. The aim of this study was to determine the association of this signature with peripheral blood mononuclear cell (PBMC) subpopulations and its specificity for sJIA as compared to related conditions.
125 patients with JIA (18 sJIA and 107 non-sJIA) and 29 controls were studied. PBMC were isolated and analyzed for multiple surface antigens by flow cytometry and for gene expression profiles. The proportions of different PBMC subpopulations were compared among sJIA, non-sJIA patients and controls and subsequently correlated with the strength of the erythropoiesis signature. Additional gene expression data from patients with familial hemophagocytic lymphohistiocytosis (FHLH) and from a published sJIA cohort were analyzed to determine if the erythropoiesis signature was present.
Keywords: Patient Vs Control, reassassment of phenotype
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| Overall design |
Peripheral blood samples were obtained from 29 healthy children and 125 children with JIA . RNA was purified from Ficoll-isolated mononuclear cells, fluorescently labeled and then hybridized to Affymetrix U133 Plus 2.0 GeneChips. Data were analyzed using ANOVA at a 5% false discovery rate threshold after Robust Multi-Array Average pre-processing and Distance Weighted Discrimination normalization.
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| Contributor(s) |
Hinze CH, Barnes MG, Fall N, Griffin TA, Thompson SD, Glass DN, Colbert RA, Grom AA |
| Citation(s) |
20576155 |
| |
| Submission date |
Apr 26, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Michael Barnes |
| E-mail(s) |
michael.barnes@cchmc.org
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| Phone |
513-636-1319
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| Organization name |
Cincinnati Childrens Hospital Medical Center
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| Department |
Rheumatology
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| Lab |
Dr. David Glass
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| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (154)
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| Relations |
| BioProject |
PRJNA125963 |
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