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| Status |
Public on Mar 29, 2023 |
| Title |
DNA methylation changes in SHRSP rat offspring due to maternal protein restriction are reprogrammed by postnatal nutritional environments |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We previously demonstrated that maternal protein restriction during pregnancy increased salt sensitivity in their offspring during adulthood, involving alterations in renal DNA methylation status. In this study, we used DNA methylation arrays to comprehensively analyze the DNA methylation status of the renal promoter regions and the effects of postnatal protein intake on DNA methylation. We fed SHRSP dams a normal diet or a low-protein diet during pregnancy, and their 4-week-old offspring were fed a normal diet or a high-/low-protein diet for two weeks. We found that the methylation status of 2,395 differentially methylated DNA regions was reprogrammed, and 34 genes were reset by different levels of postnatal protein intake. Among these genes, Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1 are involved in renal disease and blood pressure regulation. Our findings indicate that postnatal nutritional interventions can potentially reprogram epigenetic changes and provide novel therapeutic and preventive epigenetic targets for salt-sensitive hypertension.
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| Overall design |
In the study presented here, 12 samples were analyzed. Methylation of DNA in rat kidneys was analyzed. CN, 20%-Casein diet; LP, 9%-Casein diet, HP, 40%-Casein diet. * AIN-76 prescription (Oriental Yeast Co., Ltd., Tokyo, Japan).
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| Contributor(s) |
Ando C, Miyoshi M, Furukawa K, Li X, Jia H, Kato H |
| Citation(s) |
37125041 |
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| Submission date |
Oct 10, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Chika Ando |
| Organization name |
University of Tokyo
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| Street address |
1-1-1
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| City |
Yayoi, Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
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| Platforms (1) |
| GPL21859 |
065989 Arraystar Rat RefSeq Promoter Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA889039 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215182_RAW.tar |
220.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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