 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 13, 2022 |
| Title |
Charting the development of Drosophila leg sensory organs at single-cell resolution |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To respond to the world around them, animals rely on the input of a network of sensory organs distributed across the body. A single individual will generally express several distinct classes of sensory organ, each bearing features that appear to enhance the detection of specific stimuli, such as strain, pressure, or light. The features that underlie this specialization relate both to the neurons housed within a sensory organ and to the various accessory cells that they are composed of. This diversity of cell types, both within and between sensory organs, raises two fundamental questions: (1) How is this diversity generated during development? And (2) What makes these cell types distinct from one another? To address these questions, we performed single-cell RNA-sequencing on a region of the pupal male Drosophila melanogaster foreleg that displays a wide variety of functionally and structurally distinct sensory organs: the first tarsal segment. Our work characterizes the cellular landscape in which the sensory organs reside; identifies a novel glia cell type that appears to contribute towards the construction of the neural lamella; resolves and validates a combinatorial transcription factor code unique to each of mechanosensory, sex comb, and campaniform sensilla neurons, as well as four distinct gustatory receptor neuron classes; matches receptors and membrane channels to specific neurons; and characterizes the transcriptomic differences between and within sensory organ support cell types. Collectively, our work identifies core genetic features of a variety of sensory organs and provides a rich, annotated resource for studying their development and function.
|
| |
|
| Overall design |
First tarsal segment cells from pupal male Drosophila melanogaster (DGRP line RAL-517) were isolated separately at 24h and 30h after puparium formation
|
| |
|
| Contributor(s) |
Hopkins BR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 08, 2022 |
| Last update date |
Oct 15, 2022 |
| Contact name |
Ben Hopkins |
| E-mail(s) |
brhopkins92@gmail.com
|
| Organization name |
UC Davis
|
| Department |
Evolution & Ecology
|
| Street address |
One Shields Avenue
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (2) |
| GSM6620796 |
First tarsal segment, male, 24h APF, scRNA-seq |
| GSM6620797 |
First tarsal segment, male, 30h APF, scRNA-seq |
|
| Relations |
| BioProject |
PRJNA888359 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215073_RAW.tar |
212.5 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
