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| Status |
Public on Oct 03, 2022 |
| Title |
Influence of Staphylococcus aureus strain background on Sa3 phage life cycle switches |
| Organism |
Staphylococcus aureus |
| Experiment type |
Other
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| Summary |
Staphylococcus aureus (S. aureus) is a known pathogen able to infect humans and animals. Human S. aureus isolates are often associated with carriage of Sa3int prophages combined with loss of beta-hemolysin production due to gene disruption, whereas animal isolates are positive for beta-hemolysin associated with absence of Sa3int prophages. Sa3int prophages are known to contribute to staphylococcal fitness and virulence in human host by providing human-specific virulence factors encoded on the prophage genome. Strain-specific differences in regard to phage transfer, lysogenization and induction are attributable to yet unknown staphylococcal factors specifically influencing prophage gene expression. In this work we used tagRNA-sequencing approach to specifically search for these unknown host factors and differences in prophage gene expression. For this purpose, we established a workflow revealing the first direct comparison for differential gene expression analysis on two distinct single-lysogenic S. aureus isolates. Further, global gene expression patterns were investigated in two S. aureus isolates upon mitomycin C treatment and compared to uninduced conditions. This provides new insights into the tightly linked host-phage interaction network.
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| Overall design |
Samples of 8325-4phi13kana and MW2cphi13kana were grown to OD600 = 0.7 (late exponential growth phase) followed by induction with mitomycin C or without treatment as a control. Each strain and condition was performed in biological triplicates
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| Contributor(s) |
Rohmer C, Dobritz R, Wolz C |
| Citation(s) |
36366569 |
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| Submission date |
Sep 30, 2022 |
| Last update date |
Jan 09, 2023 |
| Contact name |
Christiane Wolz |
| E-mail(s) |
christiane.wolz@uni-tuebingen.de
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| Organization name |
University Tübingen
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| Street address |
Elfriede-Aulhorn-Strasse 6
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| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL24034 |
Illumina NextSeq 500 (Staphylococcus aureus) |
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| Samples (36)
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| GSM6610480 |
8325-4phi13kan, - Mitomycin C, Rep 1 [1-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610481 |
8325-4phi13kan, - Mitomycin C, Rep 2 [2-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610482 |
8325-4phi13kan, - Mitomycin C, Rep 3 [3-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610483 |
8325-4phi13kan, + Mitomycin C, Rep 1 [4-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610484 |
8325-4phi13kan, + Mitomycin C, Rep 2 [5-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610485 |
8325-4phi13kan, + Mitomycin C, Rep 3 [6-8325-4DF-phi-13Kana-delta-rep_unassigned] |
| GSM6610486 |
MW2c phi13kan, + Mitomycin C, Rep 1 [7-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610487 |
MW2c phi13kan, + Mitomycin C, Rep 2 [8-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610488 |
MW2c phi13kan, + Mitomycin C, Rep 3 [9-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610489 |
MW2c phi13kan, - Mitomycin C, Rep 1 [10-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610490 |
MW2c phi13kan, - Mitomycin C, Rep 2 [11-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610491 |
MW2c phi13kan, - Mitomycin C, Rep 3 [12-MW2c-phi-13Kana-delta-rep_unassigned] |
| GSM6610492 |
8325-4phi13kan, - Mitomycin C, Rep 1 [1-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610493 |
8325-4phi13kan, - Mitomycin C, Rep 2 [2-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610494 |
8325-4phi13kan, - Mitomycin C, Rep 3 [3-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610495 |
8325-4phi13kan, + Mitomycin C, Rep 1 [4-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610496 |
8325-4phi13kan, + Mitomycin C, Rep 2 [5-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610497 |
8325-4phi13kan, + Mitomycin C, Rep 3 [6-8325-4DF-phi-13Kana-delta-rep_TSS] |
| GSM6610498 |
MW2c phi13kan, + Mitomycin C, Rep 1 [7-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610499 |
MW2c phi13kan, + Mitomycin C, Rep 2 [8-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610500 |
MW2c phi13kan, + Mitomycin C, Rep 3 [9-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610501 |
MW2c phi13kan, - Mitomycin C, Rep 1 [10-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610502 |
MW2c phi13kan, - Mitomycin C, Rep 2 [11-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610503 |
MW2c phi13kan, - Mitomycin C, Rep 3 [12-MW2c-phi-13Kana-delta-rep_TSS] |
| GSM6610504 |
8325-4phi13kan, - Mitomycin C, Rep 1 [1-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610505 |
8325-4phi13kan, - Mitomycin C, Rep 2 [2-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610506 |
8325-4phi13kan, - Mitomycin C, Rep 3 [3-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610507 |
8325-4phi13kan, + Mitomycin C, Rep 1 [4-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610508 |
8325-4phi13kan, + Mitomycin C, Rep 2 [5-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610509 |
8325-4phi13kan, + Mitomycin C, Rep 3 [6-8325-4DF-phi-13Kana-delta-rep_PSS] |
| GSM6610510 |
MW2c phi13kan, + Mitomycin C, Rep 1 [7-MW2c-phi-13Kana-delta-rep_PSS] |
| GSM6610511 |
MW2c phi13kan, + Mitomycin C, Rep 2 [8-MW2c-phi-13Kana-delta-rep_PSS] |
| GSM6610512 |
MW2c phi13kan, + Mitomycin C, Rep 3 [9-MW2c-phi-13Kana-delta-rep_PSS] |
| GSM6610513 |
MW2c phi13kan, - Mitomycin C, Rep 1 [10-MW2c-phi-13Kana-delta-rep_PSS] |
| GSM6610514 |
MW2c phi13kan, - Mitomycin C, Rep 2 [11-MW2c-phi-13Kana-delta-rep_PSS] |
| GSM6610515 |
MW2c phi13kan, - Mitomycin C, Rep 3 [12-MW2c-phi-13Kana-delta-rep_PSS] |
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| Relations |
| BioProject |
PRJNA885804 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214523_RAW.tar |
960.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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