Expression profiling by high throughput sequencing
Summary
To investigate the mode of action of ccc_R08, a first-in-class orally available HBV cccDNA inhibitor, we designed and implemented two orthogonal and complementary approaches: a forward pharmacology approach and a reverse pharmacology approach. Bioinformatics analysis integrating biological knowledge with the observations from both approaches offered us preliminary insights into the mode of action of ccc_R08.
Overall design
In the forward pharmacology approach, we characterized the transcriptome profiles of HBV-infected PHH treated with the active compound ccc_R08, a less active compound ccc_R07 derived from the same chemical series with an identical backbone and with side-chain modifications, and DMSO as control.
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