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| Status |
Public on Nov 30, 2023 |
| Title |
OCA-B/Pou2af1 is sufficient to promote CD4+ T cell memory and prospectively identifies memory precursors [bulk RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sun et al. use T cell conditional knockout and ectopic expression to show that OCA-B is required for, and sufficient to promote, CD4+ memory T cell formation in vivo, and can be used to prospectively identify effector T cells with enhanced memory potential.
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| Overall design |
CD4+ T cells were purified from female Ly5.1 SMARTA and Ly5.1/5.2 SMARTA mice, and separately transduced with pMIG-OCA-B or empty vector (EV), respectively. On the following day, transduced GFP+ cells were sorted, 20K of each combined 1:1, and injected intravenously into C57BL/6J recipients mice. 24h later, each recipient mouse was injected intraperitoneally with 2x10^5 pfu LCMVArm. After eight days,CD4+GFP+Ly5.1+Ly5.2+ (EV) and GFP+Ly5.1+ (OCA-B-expressing) cells were sorted and used for RNA purification. Then RNA samples are applied for bulk RNAseq.
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| Contributor(s) |
Tantin D, Sun W, Kim H, Perovanovic J, Hughes EP |
| Citation(s) |
38386711 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI100873 |
Role of Transcription Coactivator OCA-B in Gene Poising and Immunological Memory |
UNIVERSITY OF UTAH |
Dean Tantin |
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| Submission date |
Sep 27, 2022 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Dean Tantin |
| E-mail(s) |
dean.tantin@path.utah.edu
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| Phone |
(801) 587-3035
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| Organization name |
University of Utah
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| Department |
Department of Pathology
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| Street address |
15 North Medical Drive East
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE214310 |
OCA-B/Pou2af1 is sufficient to promote CD4 memory and prospectively identifies memory precursors |
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| Relations |
| BioProject |
PRJNA884832 |
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