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Series GSE214151 Query DataSets for GSE214151
Status Public on May 08, 2024
Title HBcore-specific CXCR6+CD8 T cells during persistent HBV replication in mice lose their effector function and have high CREM activity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We investigated molecular profiles of CD8 T cells in persistent experimental Hepatitis B virus (HBV) infection. To this end, we established a preclinical in vivo model where HBV-replicating hepatocytes were cleared by virus-specific immunity after infection with 107 IU Ad-HBV, whereas persistent HBV replication developed after infection with 108 IU Ad-HBV, revealed by high serum HBeAg levels, high numbers of HBV genome copies and of HBcorepos hepatocytes in liver tissue. To overcome variable surface expression of the T cell receptor during chronic infection and unequivocally identify HBV-specific CD8 T cells, we adoptively transferred naïve CD45.1+HBcore-specific CD8 T cells from Cor93 transgenic mice (HBcoreCD8 T cells) the day before Ad-HBV infection. After clearance of HBV-replicating hepatocytes (d44 p.i.), liver CD45.1+HBcoreCD8 T cells expressed either CXCR6 or CX3CR1, and in the spleen only CX3CR1+ CD8 T cells were detected. Next, we evaluated the transcriptional profile of FACSorted HBcoreCD8 T cells after resolved compared to persistent infection employing SmartSeq2. The resulting dataset is reported here.
 
Overall design We compared expression profiles between subtypes of HBVcore-specific CD8 T cells in acute and chronic infection in either liver or spleen, and inferred gene regulatory networks underlying the observatios.
Web link https://doi.org/10.1038/s41586-024-07630-7
 
Contributor(s) Griesser V, Zhang JD, Kumpesa N, Bosch M
Citation(s) 38987588
Submission date Sep 25, 2022
Last update date Aug 07, 2024
Contact name Jitao David Zhang
E-mail(s) jitao_david.zhang@roche.com
Phone +41616886251
Organization name F. Hoffmann-La Roche
Department Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel
Lab Pharmaceutical Sciences
Street address Grenzacherstrasse 124
City Basel
ZIP/Postal code 4070
Country Switzerland
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (40)
GSM6600256 AdHBV_resolved_CD45_1neg_CD8_mouse10_liver
GSM6600257 AdHBV_resolved_CD45_1neg_CD8_mouse10_spleen
GSM6600258 AdHBV_persistent_CD45_1neg_CD8_mouse11_liver
Relations
BioProject PRJNA884135

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE214151_20220925-RocheTUM-HBVTcells-counts.gct.gz 1.2 Mb (ftp)(http) GCT
GSE214151_20220925-RocheTUM-HBVTcells-featureAnnotation.tsv.gz 677.7 Kb (ftp)(http) TSV
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Processed data are available on Series record
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