 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 16, 2022 |
| Title |
Reevaluation of Piezo1 as a gut RNA sensor |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Piezo1 is a stretch-gated ion channel required for mechanosensation in many organ systems. Recent provocative findings describe a new role for Piezo1 in the gut, suggesting that it is a sensor of microbial single-stranded RNA (ssRNA) rather than mechanical force. If true, this would redefine the scope of Piezo biology. Here, we sought to replicate the central finding that fecal ssRNA is a natural agonist of Piezo1. While we observed that fecal extracts and ssRNA stimulate calcium influx in certain cell lines, this response was independent of Piezo1. Additionally, sterilized dietary extracts devoid of gut biome RNA showed similar cell line-specific stimulatory activity to fecal extracts. Together, our data highlight potential confounds inherent to gut-derived extracts, exclude Piezo1 as a receptor for ssRNA in the gut, and support a dedicated role for Piezo channels in mechanosensing.
|
| |
|
| Overall design |
Nuclei from RIN14B cells were isolated using FACS and single nucleus RNA-seq were performed with 10x Genomics method.
|
| Web link |
https://elifesciences.org/articles/83346
|
| |
|
| Contributor(s) |
Alec N, Alexander C |
| Citation(s) |
36382883 |
| |
| Submission date |
Sep 21, 2022 |
| Last update date |
Feb 15, 2023 |
| Contact name |
Minh Q Nguyen |
| Organization name |
NIDCR/NIH
|
| Street address |
9000, Rockville Pike
|
| City |
Bethesda |
| State/province |
MARYLAND |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA882906 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213903_RAW.tar |
940.0 Kb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
