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| Status |
Public on Jun 22, 2023 |
| Title |
Cancer associated fibroblasts play an important role in prostate cancer progression [ATAC-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To understand the cell types changes in prostate cancer progression, we carried out scRNA-seq of prostates from different kinds of genetic mouse model. After that, we found fibroblasts were changed obviously during prostate cancer progression. To confirm the function of the fibroblasts, LNCap was used to cocultured with all kinds of fibroblasts in control or ADT condition. To find out the key enviroment factors induced firoblast changing, RNA-seq, ATAC-seq and ChIP-seq of fibroblast in different treatment were taken. And finally, ADT, TGF-β1, and sox4 were confirmed as contributors of the stroma cell changes, which provided new strategies for prostate cancer treatment.
scRNA-seq were used to find out the cell lines diverisity of prostate cancer progressiom. RNA-seq of LNCap cocultured with different fibroblasts were taken to identify the pathway fibroblasts induced. RNA-seq, ATAC-seq and ChIP-seq of fibroblasts were carried out to find out the reason of fibroblast changes.
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| Overall design |
Examination of chromatin accessibility in different kinds of CAFs.
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| Contributor(s) |
Qin J, Wang H |
| Citation missing |
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| |
| Submission date |
Sep 19, 2022 |
| Last update date |
Jun 25, 2023 |
| Contact name |
Hanling Wang |
| E-mail(s) |
hlwang@sinh.ac.cn
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| Organization name |
Shanghai Institute of Nutrition and Health, Chinese Academy of Science
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| Street address |
Shanghai
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE213670 |
Cancer associated fibroblasts play an important role in prostate cancer progression |
|
| Relations |
| BioProject |
PRJNA882047 |
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