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| Status |
Public on Nov 08, 2022 |
| Title |
Global DNA methylation analysis of cancer-associated fibroblasts reveals extensive epigenetic rewiring linked with RUNX1 upregulation in breast cancer stroma [ly6c] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA seq to charachterize the transcriptome of specific cancer associates fibroblasts (pCAFs) sub-populations (Ly6c+ vs Ly6c- pCAFs)
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| Overall design |
4T1 cells were injected orthotopcally into the mammary fat pad of balb\c mice, tumors were harvested and digested after reaching a size of 1 cm. Ly6c+ and Ly6c- pCAF were collected by fluorescence-activated cell sorting (FACS) based on negative selection excluding EpCAM-, CD45-, and Ter119- and positive selection using PDPN. To subset the pCAFs we used anti-Ly6C antibody to separate between the negative and positive subsets.
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| Contributor(s) |
Halperin C, hey J, Plass C, Shouval RS |
| Citation(s) |
36287637 |
| |
| Submission date |
Sep 10, 2022 |
| Last update date |
Nov 08, 2022 |
| Contact name |
Coral Halperin |
| E-mail(s) |
coral.halperin@weizmann.ac.il
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| Organization name |
Weizmann institute of sciences
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| Lab |
Scherz-Shouval
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| Street address |
Herzel 234
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| City |
rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE195865 |
Global DNA methylation analysis of cancer-associated fibroblasts reveals extensive epigenetic rewiring linked with RUNX1 upregulation in breast cancer stroma |
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| Relations |
| BioProject |
PRJNA879056 |
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