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| Status |
Public on Nov 01, 2023 |
| Title |
Expression of miRNAs in patient-derived cancer models and models of drug resistance |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We examined whether tumor samples can be stratified according to miRNA expression profiles.
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| Overall design |
We performed miRNA sequencing in 12 patient derived cancer models (PDCMs, organoids/spheroids) and models of drug resistance. These included the melanoma WM1366 cell line and both related BRAF/MEK inhibitor resistant daughter cell lines WM1366VCi and WM1366DTi. In addition, we included 8 patient-derived 3D cancer models, represented by two spheroid models (CRC02-SP, CRC03-SP) and three organoid models (CRC01-OG, CRC03-OG, CRC04-OG) from patients with CRC. In addition, we studied one organoid model from a patient with cancer of unknown primary (CUP01-OG), and one spheroid culture from a patient with teratoma (TER01-SP). To also test whether and how miRNA expression differs between related samples, we included a normal colon mucosa organoid culture (CRC01NM-OG) for the CRC patient 1 (CRC01-OG). In addition, we created an irinotecan resistant subclone of the CRC patient 2 spheroid culture (CRC02-SP) by constantly adding increasing concentrations of SN38 for at least 4 passages.
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| Contributor(s) |
Wurm AA, Glimm H |
| Citation(s) |
37734378 |
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| Submission date |
Sep 08, 2022 |
| Last update date |
Nov 02, 2023 |
| Contact name |
Alexander Arthur Wurm |
| E-mail(s) |
alexander.wurm@nct-dresden.de
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| Organization name |
NCT Dresden
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| Street address |
Fetscherstrasse 74
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| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA877846 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212897_Processed_Data_All_Samples.xlsx |
138.3 Kb |
(ftp)(http) |
XLSX |
| GSE212897_RAW.tar |
90.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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