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| Status |
Public on Sep 07, 2022 |
| Title |
Chromatin accessibility of transcription factor Bach2 in mouse NK cells (ATAC-seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Transcription factor Bach2 has been reported to regulate the development and function of multiple immune cells including T cells and B cells. As a transcription repressor, Bach2 plays its role by repressing the expression of downstream targets. We are interested in whether Bach2 will affect NK cell development and what's the relationship between Bach2 and other transcription factors in regulating NK cell function. Here we try to explore the chromatin accessibility of Bach2 by ATAC-seq. We compared enriched peaks in mouse NK cell from Bach2 conditional knockout mice and control mice.
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| Overall design |
Fresh splenic NK cells were sorted into CD3-CD19-NK1.1+NKp46+ cells. ATAC-seq libraries were generated using the omni-ATAC protocol. All ATAC libraries were barcoded, pooled and sequenced using 75bp paired-end read to a depth of at least 30 million.
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| Contributor(s) |
Li S, Fan C, Xing X, Wang T, Yokoyama WM |
| Citation(s) |
36190189 |
| |
| Submission date |
Sep 06, 2022 |
| Last update date |
Oct 27, 2022 |
| Contact name |
Shasha Li |
| E-mail(s) |
li.shasha@wustl.edu
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| Organization name |
Washington University in St.Louis
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| Department |
Rheumatology
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| Lab |
Wayne Yokoyama
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| Street address |
660 S Euclid Avenue
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| City |
St.Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA877357 |
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