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| Status |
Public on Oct 01, 2022 |
| Title |
Analyze the mechanism of polyploidization of proximal tubular cells (PTC) in the unilateral ischemia reperfusion injury model of acute kidney injury (AKI) in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that PTC undergo polyploidization immediately after AKI induced by ischemia. To identify the mechanism controlling polyploidization of PTC, we generated single-cell RNA sequencing (scRNAseq) datasets from healthy mouse kidneys, 2 and 30 days after ischemia reperfusion injury. We found that polyploid PTC become hypertrophic and undergo polyploidization in a YAP1-related manner. In particular, YAP1 controls polyploidization of PTC via E2f7, E2f8 and Akt1. We confirmed these findings by knocking-down those genes in vitro.
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| Overall design |
Identify and characterize the mechanism controlling the polyploidization process of PTC after Acute Kidney Injury
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| Contributor(s) |
De Chiara L, Semeraro R, Lazzeri E, Romagnani P |
| Citation(s) |
36195583, 37642236 |
| |
| Submission date |
Aug 29, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Roberto Semeraro |
| E-mail(s) |
robe.semeraro@gmail.com
|
| Organization name |
Università degli studi di Firenze
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| Department |
Medicina Sperimentale e Clinica
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| Lab |
206, secondo piano, Cubo 3
|
| Street address |
Viale Pieraccini, 6
|
| City |
Firenze |
| ZIP/Postal code |
50139 |
| Country |
Italy |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA874740 |
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