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| Status |
Public on Oct 31, 2022 |
| Title |
Transcriptome analysis of in vitro hematopoietic stem cells (HSC) and stage 3 (ST3) NK cell progenitors cells expressing a TXNIP-specific shRNA. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function of TXNIP in the regulation of natural killer (NK) cell differentiation, we established in vitro NK cell differentation cultures starting with cordblood derived HSC in which TXNIP has been knocked down with shRNA by lentiviral transduction. We then performed transcriptome analysis of the HSC population at day 3 and ST3 population at day 7.
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| Overall design |
mRNA profile of HSC (d3) and ST3 (d7) transduced with shTXNIP in comparison to control transduced cells
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| Contributor(s) |
Persyn E, Van Loocke W, Leclercq G |
| Citation(s) |
36232644 |
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| Submission date |
Aug 25, 2022 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Georges Leclercq |
| E-mail(s) |
georges.leclercq@ugent.be
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| Organization name |
Ghent University
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| Street address |
C. Heymanslaan
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| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (20)
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| GSM6507494 |
HSC, Control, Day 3, rep 4 |
| GSM6507495 |
HSC, Control, Day 3, rep 5 |
| GSM6507496 |
HSC, shTXNIP, Day 3, rep 1 |
| GSM6507497 |
HSC, shTXNIP, Day 3, rep 2 |
| GSM6507498 |
HSC, shTXNIP, Day 3, rep 3 |
| GSM6507499 |
HSC, shTXNIP, Day 3, rep 4 |
| GSM6507500 |
HSC, shTXNIP, Day 3, rep 5 |
| GSM6507501 |
ST3, Control, Day 7, rep 1 |
| GSM6507502 |
ST3, Control, Day 7, rep 2 |
| GSM6507503 |
ST3, Control, Day 7, rep 3 |
| GSM6507504 |
ST3, Control, Day 7, rep 4 |
| GSM6507505 |
ST3, Control, Day 7, rep 5 |
| GSM6507506 |
ST3, shTXNIP, Day 7, rep 1 |
| GSM6507507 |
ST3, shTXNIP, Day 7, rep 2 |
| GSM6507508 |
ST3, shTXNIP, Day 7, rep 3 |
| GSM6507509 |
ST3, shTXNIP, Day 7, rep 4 |
| GSM6507510 |
ST3, shTXNIP, Day 7, rep 5 |
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| This SubSeries is part of SuperSeries: |
| GSE212025 |
Transcriptome analysis of in vitro hematopoietic stem cells (HSC) and stage 3 (ST3) NK cell progenitors cells |
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| Relations |
| BioProject |
PRJNA873583 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212024_countMatrix_Eva.txt.gz |
776.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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